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目的:比较2组不同程度颈部淋巴节结转移的初诊鼻咽癌组织在蛋白质组水平上的表达差异。方法:将52例2010-06-01-2011-03-31中山大学肿瘤防治中心初诊鼻咽癌患者,按照颈部淋巴结转移程度分为A组(N0~N1)和B组(N2~N3),分别收集每组患者的肿瘤组织,提取总蛋白,进行双向凝胶电泳分离和质谱分析,利用UMAX PowerLook1100透射扫描仪获取凝胶图像,应用PDQuest 7.1.0软件包进行图像分析,选取凝胶图谱中差异表达>1.5倍且P<0.01的蛋白质斑点,应用基质辅助激光解吸/电离-飞行时间质谱仪(matrix assisted laser desorption ionization time of flight/mass spectrometry,MALDI-TOF/MS)进行蛋白质的肽指纹图谱鉴定。结果:在2组颈部淋巴节结转移程度不同的初诊鼻咽癌患者肿瘤组织间,共有7个蛋白质点强度差异≥1.5倍且P<0.01。经质谱分析鉴定发现,B组中的表达量比在A组中的表达量,Stathmin升高>5倍,缺氧诱导因子1-α抑制剂升高>10倍。结论:Stathmin和缺氧诱导因子1-α抑制剂在高淋巴结转移的鼻咽癌组织中表达增加,提示其在肿瘤发生发展机制中的作用可能与淋巴结转移有关。
OBJECTIVE: To compare the differences in proteome expression of newly diagnosed nasopharyngeal carcinoma between two groups of cervical lymph node nodal metastases. METHODS: Fifty-two patients with newly diagnosed nasopharyngeal carcinoma at the Cancer Center of Sun Yat-sen University from 2010-06-01-2011-03-31 were divided into group A (N0~N1) and group B (N2~N3) according to the degree of cervical lymph node metastasis. The tumor tissues were collected from each group, total protein was extracted, separated by two-dimensional gel electrophoresis, and analyzed by mass spectrometry. The gel images were acquired using UMAX PowerLook 1100 transmission scanner. The image was analyzed using PDQuest 7.1.0 software package and the gel profile was selected. Protein spots with >1.5-fold and P<0.01 differentially expressed, peptide fingerprinting of proteins using matrix assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) Atlas identification. RESULTS: There was a difference of 7 protein spots between the tumor tissues of newly diagnosed nasopharyngeal carcinoma patients with different degrees of lymph node nodal metastasis in the two groups (≥1.5 times and P<0.01). Through mass spectrometry analysis, it was found that the expression level in group B was higher than that in group A, Stathmin was increased by 5 times, and the inhibitor of hypoxia-inducible factor 1-alpha was increased by 10 times. CONCLUSION: Stathmin and HIF-1α inhibitors increase in NPC tissues with high lymph node metastasis, suggesting that the role of Stathmin and HIF-1α inhibitors may be related to lymph node metastasis.