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目的探讨孕期膳食对子代肥胖和肥胖相关基因脂肪酸合成酶(FAS)和磷酸烯醇丙酮酸羧激酶(PEPCK)表达的影响。方法将Wistar孕鼠随机分为2组,分别食用标准饲料和高蛋白质饲料。所产雄性子代分为对照组和高蛋白质组。交叉哺乳,断乳后用标准饲料喂养至成年时,从对照组中随机抽取一部分大鼠(高脂对照组)与高蛋白质组的大鼠均喂饲高脂饲料,高脂诱导肥胖至实验结束。分期处死大鼠取材,应用荧光定量PCR法检测褐色脂肪中FAS及PEPCK mRNA的表达丰度。结果孕期高蛋白质饲料可降低子代断乳及高脂诱导肥胖后的体重及肥胖率(P<0.05);高蛋白质组褐色脂肪组织中PEPCK mRNA的表达持续低于对照值和高脂对照组,而FAS mRNA在诱导肥胖前、后分别低于对照组和高脂对照组。结论孕期适当增加蛋白质摄入,可通过程序性降低子代FAS和PEPCK基因的表达,预防子代成年高脂诱导肥胖的发生。
Objective To investigate the effects of pregnancy diet on the expression of obesity-related genes fatty acid synthase (FAS) and phosphoenolpyruvate carboxykinase (PEPCK) in offspring. Methods Wistar pregnant rats were randomly divided into two groups, which were fed with standard diet and high protein diet respectively. Produced male offspring into control and high proteome. Cross-feeding, weaned after standard feed to adulthood, from the control group were randomly selected part of the rats (high-fat control group) and high proteome rats were fed high-fat diet, high fat-induced obesity to the end of the experiment . The rats were sacrificed by staging, and the expression abundance of FAS and PEPCK mRNA in brown fat were detected by real-time PCR. Results High protein diet during pregnancy could reduce the body weight and obesity rate of offspring and fat-induced obesity (P <0.05). The expression of PEPCK mRNA in brown adipose tissue of high-protein group continued to be lower than that of control group and high-fat control group, However, FAS mRNA was lower than the control group and the high-fat control group before and after the induction of obesity. Conclusions Proper increase of protein intake during pregnancy can prevent the occurrence of fat-induced adult hyperlipidemia by reducing the expression of FAS and PEPCK gene in progeny programmatically.