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目的应用四氯化碳造成兔肝硬化模型,并建立动物门静脉给药系统。方法雄性新西兰大白兔给予皮下注射四氯化碳,每周两次,12周后检测肝脏病理改变及肝组织羟脯氨酸含量。通过小肠系膜静脉末端进行门静脉插管术,将导管引致动物腹部皮下,通过经皮穿刺方法进行门静脉给药。结果四氯化碳注射12周后,兔肝组织出现典型的假小叶,肝组织羟脯氨酸含量较正常组升高3.5倍以上。门静脉给药系统可在体内维持达12周,但接头在体内易于老化破损。结论应用四氯化碳皮下注射方法成功地建立了一种兔肝硬化动物模型,并建立了一种经济简便的动物门静脉给药体系。
Objective To apply carbon tetrachloride to induce rabbit cirrhosis model and establish animal portal vein delivery system. Methods Male New Zealand white rabbits were given subcutaneous injection of carbon tetrachloride twice a week. After 12 weeks, the liver pathological changes and the content of hydroxyproline in liver tissue were detected. Portal vein catheterization was performed at the end of the small mesenteric vein and the catheter was administered subcutaneously to the abdomen of the animal for intravenous administration by percutaneous puncture. Results After the CCl 4 injection for 12 weeks, the rabbit liver tissue showed typical pseudolobules, and the content of hydroxyproline in the liver tissue increased more than 3.5 times than that in the normal group. The portal vein administration system can be maintained in the body for up to 12 weeks, but the connector is prone to age breakage in the body. Conclusion An animal model of hepatic cirrhosis was successfully established by subcutaneous injection of carbon tetrachloride. A simple and convenient animal portal vein administration system was established.