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以沈阳生态站长期定位试验为研究平台,采用传统氯仿熏蒸方法和现代PCR-DGGE技术探讨了长期不同施肥制度对土壤微生物生物量碳和氮及细菌群落结构的影响.结果表明:在整个试验期,土壤微生物生物量碳和氮的动态变化趋势基本相同;长期施用有机肥可显著提高土壤有机碳和土壤微生物生物量碳和氮含量,而长期施用化肥明显降低土壤pH,土壤微生物生物量碳和氮含量也显著降低.DGGE图谱表明:不同施肥处理的细菌16S rDNA多数条带分布相同,28条带中有18条为共有条带,说明潮棕壤中细菌类群较稳定,但其数量受到施肥的影响;长期施用有机肥促进潮棕壤细菌群落结构的多样性,而施用化肥处理则降低了其多样性.
Taking the long-term experiment of Shenyang Ecological Station as the research platform, the effects of long-term different fertilization systems on soil microbial biomass C and N and bacterial community structure were discussed by traditional fumigation methods and PCR-DGGE method.The results showed that during the whole experiment period , And the trend of soil microbial biomass C and N was basically the same. Long-term application of organic fertilizer could significantly increase soil microbial biomass carbon and nitrogen, while long-term application of chemical fertilizer significantly decreased soil pH, soil microbial biomass carbon and The nitrogen content was also significantly reduced.DGGE maps showed that most of the 16S rDNA bands of the different fertilization treatments were the same and 18 of the 28 bands were common bands, indicating that the bacterial population in the tidal brown soil was stable but the amount was affected by fertilization The long-term application of organic manure promoted the diversity of bacterial community structure in tidal brown soil, while the application of chemical fertilizer reduced its diversity.