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萤光抗体方法是用萤光色素标记抗体(即萤光色素和抗体结合)。以此标记抗体再与抗原进行特异性结合(即抗原抗体反应),于萤光显微镜下观察其萤光现象,以资诊断。此法自1942年Coons氏等介绍以来,陆续有很多文章报导,有了很快的发展,并已广泛地应用于生物学、医学诸方面。本文就萤光抗体方法的历史发展、萤光色秦、染色方法及其在医学上的应用和排除非特异性染色的方法加以综述。历史发展 1930—1956年间有很多学者报告抗体能与某些化合物结合,结合后并不破坏其和抗原
Fluorescent antibody method is to use fluorescent pigments labeled antibody (ie fluorescent dye and antibody binding). The labeled antibody is then specifically bound to the antigen (ie, the antigen-antibody reaction), and its fluorescence is observed under a fluorescence microscope for diagnosis. Since the introduction of Coons et al. In 1942, this method has been reported in many articles one after another, with rapid development and has been widely used in biology and medicine. This article reviews the history of the development of fluorescent antibody methods, the methods of fluorescent colorimetry, staining and their applications in medicine, and the methods of excluding nonspecific staining. Historical developments 1930-1956 years, many scholars reported that antibodies can be combined with certain compounds, after the combination does not destroy its antigen