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试验采用高效液相色谱法法比较不同提取工艺对芦荟叶中没食子酸(Gallic acid)、原儿茶酸(Protocate-chuicacid)、儿茶素(Catechin)、绿原酸(Chlorogenicacid)、表儿茶素(Catechin)、咖啡酸(Caffeicacid)、阿魏酸(Ferulicacid)和芦丁(Rutin)8种多酚含量的影响。试验采用ZORBAX SB-C18色谱柱(125 mm×4.6 mm×5μm),流动相A相为乙腈;流动相B相为0.1(V/V)甲酸,梯度洗脱,流速为0.8 mL/min,DAD检测波长为280 nm,柱温为45℃。检测结果表明,不同提取工艺得到的芦荟叶粉中没食子酸、原儿茶酸、儿茶素、绿原酸、表儿茶素、咖啡酸、阿魏酸和芦丁8种芦荟叶多酚的总含量以超临界二氧化碳萃取最高,超临界二氧化碳萃取过程没有加热处理,最大程度地保留了芦荟叶多酚;该检测方法在加标为1 000~100 000μg/kg浓度范围内的回收率为98.21%~103.28%,精密度为1.29%~4.67%(n=6),方法的检出限为0.02~0.05 mg/L,检测线性范围最宽为0.01~500 mg/kg;结果表明,从芦荟叶多酚的含量及物质特性保留的角度考虑,选择芦荟叶的提取工艺以超临界二氧化碳萃取为宜。
The effects of different extraction processes on Gallic acid, Protocate-chuicacid, Catechin, Chlorogenic acid, epicatechin in aloe leaves were compared by high performance liquid chromatography (HPLC) Catechin, Caffeic acid, Ferulicacid and Rutin. The mobile phase A was acetonitrile and the mobile phase B was 0.1 (V / V) formic acid. The gradient elution was performed on a ZORBAX SB-C18 column (125 mm × 4.6 mm × 5 μm) with a flow rate of 0.8 mL / The detection wavelength was 280 nm and the column temperature was 45 ℃. The results showed that the contents of gallic acid, protocatechuic acid, catechin, chlorogenic acid, epicatechin, caffeic acid, ferulic acid and rutin in aloe vera leaf powder obtained by different extraction processes were higher The total content was the highest in supercritical carbon dioxide extraction, and the supercritical carbon dioxide extraction process did not heat treatment, to maximize the retention of aloe leaf polyphenols; the detection method in the spiked range of 1000 ~ 100 000μg / kg recovery was 98.21 The detection limit was 0.02 ~ 0.05 mg / L, the linear range was 0.01 ~ 500 mg / kg. The results showed that the detection limit of Aloe vera The content of leaf polyphenols and material properties retention point of view, the selection of aloe leaf extraction process to supercritical carbon dioxide extraction is appropriate.