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目的从基因水平了解丽水市艾滋病病毒(HIV)感染长期不进展者(LTNPs)与典型进展者(TPs)的人类白细胞抗原(HLA)-A、HLA-B和HLA-DRB1位点等位基因频率,比较两类人群的差异,初步探索保护性基因和易感基因。方法应用聚合酶链反应-序列特异性引物技术(PCR-SSP),对丽水市42例LTNPs和48例TPs进行HLA-A、HLA-B和HLA-DRB1等位基因分型。结果共鉴定出9个HLA-A等位基因,16个HLA-B等位基因,12个HLA-DRB1等位基因。HLA-A*02频率LTNPs组(44.05%)高于TPs组(20.83%)(P<0.01),而A*11频率则是LINPs组(20.24%)低于TPs组(42.71%)(P<0.01);HLA-B*40频率LTNPs组(17.86%)低于TPs组(28.13%)(P>0.05);HLA-DRB*15频率LTNPs组(5.95%)低于TPs组(29.17%)(P<0.01);LTNPs组HLA基因座纯合子频率(15.87%)低于TPs组(33.33%)(P<0.01)。结论在该人群中,HLA-A*02等位基因可能与延缓艾滋病疾病进程相关,而HLA-A*11、B*40、DRB1*15可能加速艾滋病疾病进程。含HLA基因杂合子者对疾病显示出更好的抵御能力。
Objective To understand the allele frequencies of human leucocyte antigen (HLA) -A, HLA-B and HLA-DRB1 loci in patients with long-term non-progress (LTNPs) and typical progression (TPs) The differences between the two groups were compared to explore the protective genes and susceptible genes. Methods The genotypes of HLA-A, HLA-B and HLA-DRB1 alleles were detected in 42 LTNPs and 48 TPs in Lishui City by polymerase chain reaction-sequence-specific primer technique (PCR-SSP) Results A total of 9 HLA-A alleles, 16 HLA-B alleles and 12 HLA-DRB1 alleles were identified. The frequency of HLA-A * 02 in LTNPs group (44.05%) was higher than that in TPs group (20.83%) (P <0.01), while it was lower in LINPs group (20.24%) than in TPs group (42.71% 0.01). The frequencies of HLA-B * 40 LTNPs group (17.86%) were lower than TPs group (28.13%) (P> 0.05) P <0.01). The homozygote frequency of HLA loci in LTNPs group (15.87%) was lower than that in TPs group (33.33%) (P <0.01). Conclusion In this population, HLA-A * 02 allele may be related to delaying the progression of AIDS, while HLA-A * 11, B * 40 and DRB1 * 15 may accelerate the progression of AIDS. Heterozygotes containing HLA genes show better resistance to disease.