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应用骨髓长期培养（ＬＴＢＭＣ）法研究了再生障碍性贫血（再障）患者血浆的巨核细胞集落刺激活性（ＭＫ－ＣＳＡ）。分别用再障血浆、正常ＡＢ混合血浆和特发性血小板减少性紫癜（ＩＴＰ）患者血浆作为刺激正常骨髓巨核细胞生长的活性来源。结果显示三组培养贴壁层形成情况类似；再障组悬浮液中累积细胞数（３．１９±０．５４）×１０￣７高于对照组（２．４８±０．６０）×１０￣７，与ＩＴＰ组（２．６４±０．６４）×１０￣７比较，统计学差异不显著；再障组悬浮液中巨核系祖细胞（ＣＦＵ－ＭＫ）维持时间长。直接对正常骨髓细胞作半固体培养，发现再障组形成的巨核细胞集落数（９５．４±３５．２／２×１０￣５个细胞）高于对照组（４３．５±２０．６／２×１０￣５个细胞）。结果表明再障血浆具有较高ＭＫ－ＣＳＡ，推测再障患者可能存在着原发性ＣＦＵ－ＭＫ缺陷，血浆中ＭＫ－ＣＳＡ升高可能是机体的一种反馈性调节。
The megakaryocyte colony-stimulating activity (MK-CSA) in the plasma of patients with aplastic anemia (aplastic anemia) was studied using bone marrow long-term culture (LTBMC). Plasma from patients with aplastic anemia, normal AB mixed plasma and idiopathic thrombocytopenic purpura (ITP), respectively, was used as a source of activity to stimulate the growth of normal bone marrow megakaryocytes. The results showed that the formation of adherent layer was similar in all three groups. The cumulative cell number in the aplastic anemia group was 3.19 ± 0.54 × 10 ~ 7 higher than that in the control group (2.48 ± 0.60) × 10 ~ 7, compared with the ITP group (2.64 ± 0.64) × 10 ~ 7, the statistical difference was not significant; the megakaryocyte progenitor cells (CFU-MK) in the aplastic anemia group maintained for a long time. Directly on normal bone marrow cells for semi-solid culture and found that the formation of aplastic anemia group megakaryocyte colonies (95.4 ± 35.2 / 2 × 10 ~ 5 cells) higher than the control group (43.5 ± 20.6 / 2 × 10 ~ 5 cells). The results showed that aplastic anemia with high MK-CSA, speculated that aplastic anemia patients may have primary defects of CFU-MK, plasma MK-CSA may be a body’s feedback regulation.