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目的:研究二至丸的保肝活性部位群(70%乙醇组分)(active fractions in liver protection from Erzhi Pill,AFEP)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分成6组:正常对照组、四氯化碳损伤模型组、AFEP的高、中、低剂量组(19.8,13.2,6.6 g·kg-1)和联苯双酯组(150 mg·kg-1)。AFEP高、中、低剂量组每日ig 1次,共7 d,末次ig后除正常组外,其余所有小鼠予ip 0.1%CCl420 mL·kg-11次,16 h后处死全部小鼠,收集肝组织及血清标本,测定小鼠血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性;测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;计算肝指数并同时对肝组织进行病理学检查。结果:AFEP高、中剂量组具有明显的剂量依赖性降低CCl4致小鼠肝损伤血清ALT,AST值升高(P<0.01),降低肝匀浆中MDA的含量(P<0.01),增强SOD的活性(P<0.01)。通过病理学切片观察,AFEP各剂量组能显著改善肝组织的病理变化(P<0.05或P<0.01)。结论:AFEP对CCl4造成的小鼠急性肝损伤具有显著的保护作用,为其保肝活性的效应部位。
Objective: To study the protective effect of active fractions in liver protection from Erzhi Pill (AFEP) on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: Sixty Kunming mice were randomly divided into six groups: normal control group, carbon tetrachloride injury model group, AFEP high, medium and low dose groups (19.8, 13.2 and 6.6 g · kg -1) and biphenyl Diester group (150 mg · kg-1). AFEP high, medium and low dose group once daily ig, a total of 7 d, after the last ig except normal group, all other mice to ip 0.1% CCl420 mL · kg-11 times, 16 h after all mice were sacrificed, The liver tissue and serum samples were collected to measure the activity of serum ALT and AST in liver homogenates. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) Activity; calculate the liver index and at the same time pathological examination of liver tissue. Results: The group of high and medium doses of AFEP had obvious dose-dependent decrease of serum ALT and AST (P <0.01), the content of MDA in liver homogenate (P <0.01) and the increase of SOD Activity (P <0.01). Pathological sections were used to observe the pathological changes of liver tissue in AFEP groups (P <0.05 or P <0.01). CONCLUSION: AFEP has a significant protective effect on CCl4-induced acute liver injury in mice and is an effective site for its hepatoprotective activity.