DNAzyme相关论文
作为一种能够识别生物分子信息的器件或装置,电化学生物传感器能将感受到的信息输出转换成电信号用以实现对生物分析物快速、灵敏......
DNA电化学生物传感器广泛应用于基因组学、环境监测、药物筛选、食品分析等领域,尤以生物医药和生物领域发展迅速,具有广阔的临床......
细胞作为构成生物体的基本结构和功能单元,其生物环境中的许多特异性生物标志物与疾病的发生、分化和发展息息相关,对细胞生理功能......
几乎在每个细胞中都发生的DNA磷酸化对DNA的损伤修复具有重要的意义。作为一种具有5’-OH磷酸化的功能性酶,T4多核苷酸激酶（T4PNK）在......
G-四链体序列可与Hemin结合形成具有过氧化物酶活性的DNAzyme，被广泛应用于生物传感器的构建中。G-四链体的过氧化物酶活性高低对生......
荧光生物传感器的构建关键在于荧光探针的设计。DNAzyme作为一种核酸酶,非蛋白酶,对反应条件（如温度,pH）要求不苛刻,具有高特异性识......
单核苷酸变异（SNV）是由单个核苷酸发生置换、缺失或插入而导致的序列变异,是人类基因组中最常见的一种突变形式。大部分已知的遗传性......
近年来,由癌症及病毒感染引发的疾病发病率不断增长,已经对人类健康造成了巨大威胁,因此对这类疾病的早期精准诊断和灵敏检测具有......
核糖核酸酶H（RNase H）能特异性地水解DNA/RNA杂交链中的RNA链释放出DNA链,在DNA复制、修复及转录等重要细胞过程中发挥着重要作用。......
铅离子是一种常见的重金属离子污染物,会在人和动物体内积累且不易排出体外。铅离子在人体内富集到一定程度,会对人体的神经系统、......
重金属是有害污染物,由于其环境持久性、毒性和生物累积性,对人类和水生生态系统构成严重威胁。铅离子(Pb2+)是对人类健康和生态系......
自从核酶这一具有催化活性的RNA分子被发现以后,国内外研究者自然地将目光转向另一类生物大分子—DNA,力图寻找和证实一类具有催化......
荧光传感技术具有灵敏度高、选择性好、分析速度快等优点,在很多领域中都有着广泛的应用。然而,使用荧光传感技术对复杂样本进行定......
Hydrophobic N-acetyl-L-leucine grafted polyethylenimine as an efficient carrier for DNAzyme delivery
Aurora kinase A has been demonstrated to be involved in the malignant progression of many types of cancer,as its amplifi......
Electrochemical aptasensor for thrombin detection through blocking DNAzyme cleavage activity and amp
In this work,a simple and label-free ultrasensitive electrochemical aptasensor platform was proposed.In the presence of ......
Targeting EBV-LMP1 DNAzyme Enhances Radiosensitivity of Nasopharyngeal Carcinoma Cells by Inhibiting
The latent membrane protein 1 (LMP1),which is encoded by the Epstein-Barr virus (EBV) has been suggested to be one of th......
本研究构建了一种基于DNAzyme功能化磁性粒子和DNA自组装串联放大的UO22+可视化快速检测新方法。所构建的方法结合了磁性粒子易于......
基于功能化DNAzyme 和循环放大技术设计了一种检测DNA 的新方法，通过Exo Ⅲ和功能化DNAzyme 的双重放大作用可使荧光信号增强.实验......
Base on click chemistry and DNAzyme1,2,we constructed a cupric ion triggered DNA diode by a tandem linkage-cleavage proc......
Although considerable clinical attempts on various types of cancers have achieved,photodynamic therapy(PDT)still suffers......
G-quadruplex/hemin DNAzymes have been the focus of an intense interest recently owing to their quite unique enzyme-mimic......
DNAzymes 是有效的基因治疗药物,而低效的细胞内递送能力和不足的辅助因子阻碍了它们的实际生物应用。[1,2]金属有机框架化合物已......
γ-干扰素(IFN-γ)是人体内重要的免疫应答标志物,实现IFN-γ 的灵敏检测在相关疾病的临床诊断和治疗中具有重要意义[1,2]。本文利......
In the first part,we will introduce our recent work about the role of ribose G-quartets and how they affect the properti......
Aptazyme is a kind of nucleic acid probes which take advantage of the specific recognition of aptamer and the highly cat......
本研究利用DNAzyme功能化磁性粒子作为识别探针[1]、以辣根过氧化物酶(HRP)催化氧化3,3′,5,5′-四甲基联苯胺(TMB)作为识别信号、......
Oligonucleotides are useful tools in chemical biology for biosensing and therapeutics.The well-known examples include fu......
基因沉默被广泛应用于医学治疗中,人们不断追求更灵敏的检测方法和更安全有效的切割方法.DNAzyme 是通过体外选择过程产生的单链DN......
碱性磷酸酶(ALP)是一类存在于人体内的同工酶，能非特异性识别并水解游离的磷酸单酯键[1]。通常人体内异常的碱性磷酸酶活度与某些疾......
Preparation of Controllable DNA of Functionalized Porous Composite Material and Its Application in t
In this study,a novel multiple amplified electrochemical immunosensor was designed for highly efficient detection of mic......
Target-triggered DNA walker on QDs for DNAzyme-modulated double quenching and ultrasensitive MicroRN
MicroRNAs (miRNAs) are short (18-23 nucleotides),endogenous,single-stranded RNA molecules,which as one of an important c......
Highly Effective Colorimetric and Visual Identification of Tetrastigma hemsleyanum Using an Peroxida
Tetrastigma hemsleyanum Diels et Gilg (Vitaceae) is a rare and endangered perennial herb native to China....
酶是一类维持细胞正常生理活动的具有催化活性的蛋白质，其具备的特殊生物学活性在人的生理和代谢过程中发挥着重要作用。研究表明，某......
A cascade linkage-cleavage switch-on system triggered by cupric ion based on click chemistry and DNA
A cascade linkage-cleavage switch-on system triggered by cupric ion was established.The substrate strand was labeled......
There have been growing concerns over food-or water-borne pathogens and drug-resistant bacteria causing healthcare-a......
Sensing Mechanism and Performance of a Microfluidic Aptasensor Based on DNAzyme Catalyzed Chemilumin
Although the universal homogeneous G-quadruplex-DNAzyme based aptasensors have potentials to replace conventional bi......
c-jun是立早基因家族（immediate early genes，IEGs）中的重要成员之一，在包括癫痫在内的许多应激过程中具有重要作用。近年来，关于c-jun......
DNA酶作为一类具有催化活性的核酸已被广泛用于传感平台的构建。然而，其在基因治疗和化学治疗中作为智能药物载体却很少有报道......
DNAzyme是一种可以对金属离子特异性识别响应的功能核酸，目前已被广泛应用于生物分析和生物医学等领域中。但是，天然的DNAzyme(D......
葡萄糖作为生物体内主要的供能物质是活细胞的能量来源和新陈代谢的中间产物，浓度过高会导致肥胖和糖尿病；过低会造成低血糖症及......
锰(Mn)和铜(Cu)是人体容易接触到的两种元素,然而过量的锰或铜会对人体产生危害.由于环境中的M和Cr含量较低,而且除了它们之外还存......
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