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目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.
Objective To investigate the genetic susceptibility of the HEN-DQB1 allele of esophageal cancer in Hubei Han population from the genetic level. METHODS: Using sequence-specific primers polymerase chain reaction technique to detect unrelated healthy Hubei and Hubei 136 cases and esophageal cancer group 42 cases. Patient’s HLA-DQB1 allele. SAS system statistical software data processing. Results Hubei Han people with esophageal cancer compared with normal people, HEN-DQB1*0301 gene frequency was significantly increased (0.2976 vs 0.1875), P = 0.046, OR = 1.835 Etiological score = 0.1354); the distribution frequency of the remaining alleles of HLA-DQB1 between the two groups, HLA-DQB1*0201 (0.0833 vs 0.1016), *0301 (0.2976 vs 0.1875), *0302 (0.0595 vs 0859), *0303 (0.2381 vs 0.1875), *0304 (0.0000 vs 0.0039), *0401 (0.0714 vs 0.0469), *0402 (0.0119 vs 0.0156), *0501 (0.0357 vs 0.0703), *0502 (0.0595 vs 0.0664), *0503 (0.0119 vs 0.0195), *0504 (0.0000 vs 0.0039), *0601 (0.0595 vs 0.0781), *0602 (0.0476 vs 0.0742), *0603 (0.0000 vs 0.0078), *0604 (0.0238 vs 0.0508), and the differences were not significant. Sex. Conclusion The HLA-DQB1*0301 allele is positively associated with esophageal cancer in Hubei Han population and is a susceptibility gene.