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本实验室用地高辛 dUTP随机引物方法标记人内皮细胞特异性分子(humanendothelialspecificmo lecular 1,hESM 1)cDNA探针,并应用于ESM-1基因在肾癌中mRNA转录的检测。现报道如下。1 材料与方法1. 1 主要试剂 pET28b hESM 1重组质粒由本实验室构建和保存 [1]; 10g/L溴化乙
In our laboratory, cDNA probes of human endothelial cell specific molecule (hESM 1) were labeled with digoxin dUTP random primers and applied to the detection of ESM-1 mRNA transcription in renal cell carcinoma. Report as follows now. 1 Materials and methods 1.1 The main reagent pET28b hESM 1 recombinant plasmid constructed and preserved by our laboratory [1]; 10g / L B bromide