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目的运用傅立叶变换红外光谱仪和显微共焦拉曼光谱仪进行光谱测量,探讨宫颈癌组织与宫颈重度上皮内瘤变(CINⅢ)组织的光谱特征,为光谱测量在早期诊断宫颈癌中的使用提供临床依据。方法 2009年8～12月于我院就诊的宫颈鳞癌及CINⅢ患者各20例,患者经手术切除或活检获得病变组织,均利用傅立叶变换红外光谱仪和显微共焦拉曼光谱仪,分别进行红外光谱和拉曼光谱的测定。取其相应病变旁正常组织作为对照,也进行上述光谱的测定。被检测的组织相应分为四组:宫颈鳞癌组织20例,宫颈鳞癌旁组织20例,CINⅢ组织20例,CINⅢ旁组织20例。结果宫颈癌病变组织红外光谱和拉曼光谱与癌旁正常组织、CINⅢ组织、CINⅢ旁组织的光谱存在明显差异。红外光谱显示:除宫颈癌组织外,其余三种组织的光谱外观相似。宫颈癌组织具有特征性的971cm-1峰位,而CINⅢ组织、CINⅢ旁组织及癌旁组织,在971cm-1附近未检测到峰的出现。拉曼光谱显示:CINⅢ旁组织和癌旁组织光谱极为相似,宫颈癌组织与之不同,1639cm-1处振动峰为宫颈癌组织特征峰,CINⅢ组织和癌旁组织在1639cm-1附近未检测到峰的出现。结论癌组织的红外光谱和拉曼光谱具有特征峰。傅立叶变换红外光谱和拉曼光谱仪有望用来区分宫颈癌病变与正常宫颈组织,成为早期诊断宫颈癌的新方法。
Objective To investigate the spectral features of cervical intraepithelial neoplasia and cervical intraepithelial neoplasia (CINⅢ) by Fourier transform infrared spectroscopy (FT-IR) and microscopy co-laser Raman spectroscopy (SERS), so as to provide clinical application of spectral measurement in the early diagnosis of cervical cancer in accordance with. Methods Twenty cases of cervical squamous cell carcinoma and CINⅢpatients were treated in our hospital from August to December in 2009. All patients underwent surgical resection or biopsy to obtain the diseased tissues. Both of them were characterized by Fourier transform infrared spectroscopy and microscopy confocal Raman spectroscopy, Spectroscopy and Raman spectroscopy. Take the corresponding normal tissue next to the lesion as a control, but also the determination of the above spectrum. The tested tissues were divided into four groups: 20 cases of cervical squamous cell carcinoma, 20 cases of cervical squamous cell carcinoma, 20 cases of CINⅢ and 20 cases of CINⅢ. Results There was a significant difference in the spectra of cervical cancer lesions between IR and Raman spectra and adjacent normal tissues, CINⅢ and CINⅢ. Infrared spectroscopy showed that, except for cervical cancer tissues, the three other tissues had similar spectral appearances. Cervical cancer has a characteristic 971cm-1 peak, and CIN Ⅲ tissue, CIN Ⅲ paracancerous tissues and paracancerous tissues, no peak was detected near 971cm-1. Raman spectra showed that the paracancerous tissues of paracancerous tissues and paracancerous tissues were very similar. The cervical cancer tissues were different. The vibrational peak at 1639cm-1 was the characteristic peak of cervical cancer. CINⅢ and paracancerous tissues were not detected near 1639cm-1 The emergence of the peak. Conclusion The infrared spectrum and Raman spectrum of cancer tissue have characteristic peaks. Fourier transform infrared spectroscopy and Raman spectroscopy is expected to be used to distinguish cervical lesions and normal cervical tissue, a new method of early diagnosis of cervical cancer.