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目的 探讨银杏叶提取物 (ExtractofGinkgobilobaleaves ,EGB)的促智作用。方法 应用EGB 2 5、50、10 0mg·kg- 1,ig ,qd× 2 1d ,采用青龄鼠 (2月龄 )、老龄前期小鼠 (11月龄 )、东莨菪碱及亚硝酸钠致记忆缺失模型、反复脑缺血灌注致痴呆模型 ,从行为学、血液流变学、病理形态学、氧自由基代谢及前列腺素E2 等生化指标定量检测等方面研究了EGB的促智作用。结果 ①对青龄鼠的影响 :EGB 50、10 0mg·kg- 1可明显增强其学习能力 (避暗法 )。EGB 2 5、50mg·kg- 1可明显降低其纤维蛋白原 (Fib)含量、血浆粘度 (ηp)数值 ,缩小血栓形成系数 (TEL) ,缩短微循环滞留时间 (MST)。EGB 50mg·kg- 1可明显降低其脑组织脂质过氧化产物丙二醛 (MDA)、NO含量 ,并可增强超氧化物歧化物 (SOD)活性。②对老龄前期小鼠的影响 :EGB 2 5～ 10 0mg·kg- 1明显增强正常小鼠的学习记忆能力 ,且对东莨菪碱 ,亚硝酸钠造成的记忆获得 ,巩固障碍有不同程度的改善作用 (跳台法 )。用EGB 2 5～ 10 0mg·kg- 1可明显抑制红细胞压积 (HCT)、Fib、血液粘度 (ηb)的升高 ,缩小血细胞聚集系数 (VAL)、TEL。EGB 2 5～ 10 0mg·kg- 1可不同程度地降低其脑组织升高的MDA、NO含量 ,使脑组织降低的SOD活性升高。③对小鼠的影响 :EGB 2 5～ 10
Objective To investigate the nootropic effect of Extract of Ginkgobilobaleaves (EGB). METHODS: EGB 25, 50, 100 mg·kg-1, ig, qd×21d were used. Young mice (2 months old), presenile mice (11 months old), scopolamine and sodium nitrite caused memory deficits. Models and repeated cerebral ischemia-reperfusion induced dementia models were used to study the effect of EGB on brain function in terms of ethology, hemorheology, pathomorphology, oxygen free radical metabolism, and prostaglandin E2. Results 1 Effect on young mice: EGB 50, 100 mg·kg -1 can significantly enhance their learning ability (darking method). EGB 2 5,50mg·kg-1 can significantly reduce the fibrinogen (Fib) content, plasma viscosity (ηp) value, reduce the thrombosis factor (TEL), and shorten the microcirculation retention time (MST). EGB 50mg·kg-1 can significantly reduce the content of malondialdehyde (MDA) and NO in the brain tissue lipid peroxidation and enhance the activity of superoxide dismutase (SOD). 2 Effect on pre-old mice: EGB 25-100 mg·kg-1 significantly enhances the learning and memory ability of normal mice, and it has different degrees of improvement effect on the memory acquisition and consolidation barriers caused by scopolamine and sodium nitrite. Jumping method). With EGB 2 5-10 mg·kg-1, the increase of hematocrit (HCT), Fib, and blood viscosity (ηb) was significantly inhibited, and the hemocyte aggregation coefficient (VAL) and TEL were reduced. EGB 2 5～10 0 mg·kg - 1 can reduce the increase of MDA and NO in brain tissue to some extent, and increase the SOD activity in brain tissue. 3 Effects on mice : EGB 2 5 to 10