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目的 :建立聚合酶链反应 -序列特异性寡核苷酸探针 (PCR- SSOP)分型方法 ,并分析其实用价值。方法 :采用一对引物扩增所有 HL A- A2特异性抗原 ,用 2 3个探针和扩增产物杂交 ,探针用地高辛系统标记和检测 ,可鉴定 A2的 2 5个等位基因 ,并在中国人群 HL A- A*0 2等位基因的检测中进行应用 ,用 B淋巴母细胞株作阳性对照 ,同时与聚合酶链反应 -序列特异性引物 (PCR- SSP)检测方法作比较。结果 :采用本方法检测 HL A - A 2等位基因的结果与用 PCR- SSP方法检测的结果一致。结论 :实验证明本分型技术具有质控好、特异性强、敏感性高、费用低廉、特别是对大样本可快速鉴定结果等优点
Objective: To establish a PCR-SSOP typing method and analyze its practical value. Methods: A pair of primers were used to amplify all HLA-A2 specific antigens. The hybridization was performed with 23 probes and amplification products. The probes were labeled and detected by digoxin system. Twenty-five A2 alleles were identified, And used in the detection of HLA-A * 0 2 alleles in Chinese population. B lymphoblastoid cell line was used as a positive control and PCR-SSP detection method was compared . Results: The results of HL A - A 2 allele detection by this method were consistent with the results of PCR - SSP. Conclusion: Experiments show that the sub-type technology with good quality control, specificity, high sensitivity, low cost, especially for large samples can quickly identify the advantages