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目的探讨中国人群乳腺癌患者中BCSG1甲基化与乳腺癌发病及临床病理特征的关系。方法采用甲基化特异性PCR方法(MSP)分别检测82例乳腺癌组织、癌旁组织和78例良性病变组织BCSG1第一外显子CpG岛甲基化状态。统计分析其与乳腺癌临床病理特征之间的关系。结果各类标本中均存在去甲基化状态,住82例乳腺癌和相应癌旁组织中分别有27例(32.9%)和15例(18.3%)检测到纯合性去甲基化,78例乳腺良性病变组织中25例(32.1%)检测到纯合性去甲基化;82例乳腺癌和相应癌旁组织分别有55例(67.1%)和67例(81.7%)检测到甲基化,78例乳腺良性病变组织中53例(67.9%)检测到甲基化,均为杂合型甲基化;BCSG1完全去甲基化状态在乳腺癌旁组织与癌组织及癌旁组织与良性病变组织间差异均有统计学意义(P<0.05)。结论 BCSG1甲基化状态在不同人群中存在差异,在中国人群中,BCSG1去甲基化状态在乳腺癌发生发展中可能起到一定的作用。
Objective To investigate the relationship between BCSG1 methylation and the clinicopathological features of breast cancer in Chinese breast cancer patients. Methods The methylation status of exon CpG island of BCSG1 in 82 cases of breast cancer tissues, adjacent non-cancerous tissues and 78 cases of benign lesions was detected by methylation-specific PCR (MSP). Statistical analysis of its relationship with clinicopathological features of breast cancer. Results There were demethylation status in all kinds of specimens. Homozygous demethylation was detected in 27 cases (32.9%) and 15 cases (18.3%) in 82 cases of breast cancer and corresponding paracancerous tissues, respectively In 25 cases (32.1%) of benign breast lesions, homozygous demethylation was detected. In 82 cases of breast cancer and corresponding paracancerous tissues, 55 cases (67.1%) and 67 cases (81.7%) respectively detected methyl , Methylation was detected in 53 cases (67.9%) of 78 benign breast lesions, all of which were heterozygous methylation. The complete demethylation status of BCSG1 in breast cancer tissues and adjacent tissues The difference between benign and malignant lesions was statistically significant (P <0.05). Conclusion There is a difference in the methylation status of BCSG1 in different populations. In Chinese population, demethylation status of BCSG1 may play a role in the development of breast cancer.