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循环内皮细胞(circulating endothelial cells,CEC)是由于血管损伤而从血管壁脱落的成熟内皮细胞,其增殖、迁移和黏附形成管腔,在肿瘤新生血管形成过程中发挥重要作用。晚期恶性肿瘤患者CEC数量显著高于正常人对照者,提示CEC可作为抗血管生成药物的靶点和疗效监测的标志物。常用的检测CEC的方法有Hladovec法、Percoll密度梯度快速一步分离法、免疫磁性分离和流式细胞术等。近来流式细胞术发展了6色标记法,成为一种快速、可靠、灵敏的CEC检测方法。由于CEC与肿瘤血管发生密切相关,可在临床用于疗效监测、优化用药剂量、判断预后、评估药物血管毒性等。
Circulating endothelial cells (CECs) are mature endothelial cells that fall off from the blood vessel wall due to vascular damage. They proliferate, migrate and adhere to form the lumen, which plays an important role in tumor neovascularization. The number of CECs in patients with advanced malignancy was significantly higher than that in normal controls, suggesting that CEC can be used as a target of anti-angiogenic drugs and a marker of efficacy monitoring. Commonly used methods to detect CEC Hladovec method, Percoll density gradient rapid one-step separation, immunomagnetic separation and flow cytometry. Recently, flow cytometry has developed a six-color labeling method, which has become a fast, reliable and sensitive CEC detection method. As CEC is closely related to tumor angiogenesis, it can be used clinically to monitor the curative effect, optimize dosage, judge the prognosis and evaluate the drug’s vascular toxicity.