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目的:研究视网膜光损伤与视细胞凋亡的相互关系,以探讨视网膜光损伤的发生发展机制。方法:所有SD大鼠经循环光环境适应7d,实验前暗适应36h,分别于光照3,6,9,12,15和18h,灌流固定,摘除眼球。光镜标本在常规脱水、透明、石蜡包埋切片后,行苏木精-伊红(HE)、TUNEL法染色,光镜观察;电镜标本在树脂包埋、超薄切片、醋酸-柠檬酸铅双重染色后,透射电镜观察;应用CIAS-1000图像分析系统定量检测外核层面积和视细胞凋亡指数,所得数据作统计学分析。结果:可见视网膜出现光损伤和视细胞凋亡现象,随着光照时间的延长,视网膜光损伤逐渐加重,视细胞凋亡逐渐增多。在外核层,透射电镜观察见核染色质浓集,而无炎性反应。外核层面积和视细胞凋亡指数作相关性分析有显著意义。结论:视细胞凋亡是视网膜光损伤的重要机制;光损伤启动了视细胞凋亡的发生,外核层细胞核的丢失是视细胞凋亡的结果;视网膜光损伤与视细胞凋亡有着密不可分的联系。
Objective: To study the relationship between retinal light damage and apoptosis of retinal cells to explore the mechanism of retinal light damage. Methods: All SD rats were adapted to light for 7 days under light and 36 hours after dark acclimation. The rats were perfused and fixed at 3, 6, 9, 12, 15 and 18 hours respectively. Light microscope specimens were stained by hematoxylin-eosin (HE) and TUNEL method and observed with light microscope after routine dehydration, transparent and paraffin-embedded sections. Electron microscopy specimens were embedded in resin, ultrathin sections, acetic acid-lead citrate Double staining, transmission electron microscopy; using CIAS-1000 image analysis system quantitative detection of outer nuclear layer area and apoptosis index, the data for statistical analysis. Results: The retina appeared light damage and optic cell apoptosis phenomenon, with the extension of light time, retinal light damage gradually increased, depending on the cell apoptosis increased gradually. In the outer nuclear layer, transmission electron microscopy showed nuclear chromatin concentration, but no inflammatory reaction. The correlation between outer nuclear layer area and apoptosis index was significant. Conclusion: Apparent cell apoptosis is an important mechanism of retinal photodamage. Photodamage initiates the development of optic neuron apoptosis. The loss of nucleus in the outer nuclear layer is the result of optic cell apoptosis. The retinal photodamage is closely related to apoptosis Contact.