本文应用单克隆抗ＨＢｓＩｇＭ聚合酶链反应（ＭｃＡｂＩｇＭ／ＰＣＲ）技术对临床诊断为特发性肝病者１３１例的ＨＢＶ低水平感染检出率为４３．５０％（５７／１３１），显著高于分子杂交技术２１．３０％（２８／１３１）ｐ＜０．０５；对乙肝疫苗免疫加强接种仍无应答者检测结果显示：９例中有７例阳性，而分子杂交法仅检出４例。提示ＭｃＡｂＩｇＭ／ＰＣＲ技术对低水平ＨＢＶ感染的检测，是探讨特发性肝病的病因和研究乙肝疫苗免疫无应答原因的有效方法 In this paper, monoclonal antibody against HBsIgM polymerase chain reaction (McAbIgM / PCR) detection of 131 cases of idiopathic liver disease in patients with HBV infection was 43.50% (57/131), was significantly higher than the molecular Hybridization 21.30% (28/131) p <0.05; Hepatitis B vaccine immunostimulant vaccination still no response test results showed that: 7 of 9 cases were positive, and only 4 cases were detected by molecular hybridization. Prompt McAbIgM / PCR detection of low-level HBV infection is to explore the etiology of idiopathic liver disease and hepatitis B vaccine non-response to the immune response to an effective method