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胰岛素的结构一经阐明,各国科学家即竞相开展它的合成研究,但其中最早成功的是在1965年由我国实现的牛胰岛素全合成,随后美国和德国也报道了这方面的工作。他们使用的方法基本上仍是1906年德国化学家费歇尔合成含有18个氨基酸残基多肽时的方法。费歇尔合成多肽的方法是先把一种氨基酸的 N-端氨基用保护基屏蔽,再把另一种氨基酸 C-端羧基屏蔽,使二者反应后生成的二肽在脱去其 C-端保护基后和第三种氨基酸反应生成
Once the structure of insulin was elucidated, scientists from all over the world competed to carry out its synthetic research. However, the earliest success was the complete synthesis of bovine insulin achieved in China in 1965, and the United States and Germany also reported on this work. The method they used was basically still the method used by the German chemist Fisher in 1906 to synthesize 18 amino acid residues. Fisher’s method for peptide synthesis is to first shield the N-terminal amino group of an amino acid with a protecting group, and then mask the C-terminal carboxyl group of another amino acid so that the dipeptide produced after the reaction between the two is cleaved off. After the terminal protecting group reacts with the third amino acid