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目的：为探讨喉癌与人乳头瘤病毒（ＨＰＶ）感染的关系和ＨＰＶ在喉癌中基因组型的分布与表达。方法：应用聚合酶链反应技术（ＰＣＲ）制备非放射性探针标记物－地高辛标记ＨＰＶ共有引物探针，对１４６例喉不同病变的新鲜组织标本（喉癌６８例，喉其他病变４８例，正常喉组织３０例），进行ＨＰＶ６，１１，１６，１８，３１，３３，３５，４２，５８共９型ＨＰＶＤＮＡ感染的检测；阳性者用多重引物ＰＣＲ方法分型。结果：喉癌ＨＰＶ感染阳性率４５．６％（３１／６８），喉癌颈转移淋巴结组织阳性率２０．０％（３／１５），喉癌前病变阳性率１１．８％（２／１７），声带息肉阳性率６．３％（１／１６），１５例癌旁及１５例癌周正常喉组织均为ＨＰＶＤＮＡ阴性。ＨＰＶＤＮＡ型别分布在喉癌中以ＨＰＶ１６，１８型为主，喉良性病变中以ＨＰＶ６，１１型为主。结论：喉癌发生与ＨＰＶ感染有关。
Objective: To investigate the relationship between laryngeal cancer and human papillomavirus (HPV) infection and the distribution and expression of HPV in laryngeal cancer. METHODS: Polymerase chain reaction (PCR) method was used to prepare non-radioactive probes labeled with digoxin-labeled HPV primers, and 146 cases of fresh specimens of different laryngeal lesions (laryngeal carcinoma 68 cases, other laryngeal lesions 48 cases) 30 cases of normal laryngeal tissues were tested for HPV 6,11,16,18,31,33,35,42,58 HPV DNA infections of type 9; positive individuals were typed by multiplex primer PCR. Results: The positive rate of HPV infection in laryngeal carcinoma was 45.6% (31/68). The positive rate of lymph node metastasis in laryngeal carcinoma was 20.0% (3/15). The positive rate of laryngeal precancerous lesion was 11.8% (2/17 The positive rate of vocal cord polyps was 6.3% (1/16). 15 cases of paracancerous and 15 cases of normal laryngeal tissue were negative for HPV DNA. HPV DNA types were mainly distributed in laryngeal carcinoma and HPV16, 18 were the main types. HPV6, 11 were the main types of benign laryngeal lesions. Conclusion: Laryngeal cancer is associated with HPV infection.