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①目的探讨人乳头瘤病毒１６型和１８型（ＨＰＶ１６和ＨＰＶ１８）与喉鳞癌、喉乳头状瘤的关系。②方法应用聚合酶链反应（ＰＣＲ）技术检测３４例喉乳头状瘤、８３例喉鳞状细胞癌石蜡包埋组织中ＨＰＶ１６及ＨＰＶ１８的ＤＮＡ．③结果喉乳头状瘤及喉鳞癌组织中ＨＰＶ１６的检出率为１４．７１％和３０．１２％，两组比较差异无显著性（χ２＝３．００６，Ｐ＞０．０５）；ＨＰＶ１８在喉乳头状瘤组织中未检出，而在喉鳞癌组织中为６．０２％，两组比较，差异无显著意义（χ２＝０．０８７，Ｐ＞０．０５）。高分化喉鳞癌组织中ＨＰＶ１６的检出率显著高于中分化鳞癌（χ２＝６．０３２，Ｐ＜０．０５），而高分化与低分化、中分化与低分化鳞癌之间的差异均无显著性（χ２＝０．３５１，１．５００，Ｐ均＞０．０５）；不同病理分化喉鳞癌组织中ＨＰＶ１８的检出率差异无显著性（χ２＝０．０６０，Ｐ＞０．０５）。④结论喉鳞癌的发生与ＨＰＶ感染密切相关，尤以ＨＰＶ１６明显
①Objective To investigate the relationship between human papillomavirus types 16 and 18 (HPV16 and HPV18) and laryngeal squamous cell carcinoma and laryngeal papilloma. Methods The DNA of HPV16 and HPV18 in paraffin-embedded tissues from 34 cases of laryngeal papilloma and 83 cases of laryngeal squamous cell carcinoma were detected by polymerase chain reaction (PCR). Results The positive rates of HPV16 in laryngeal papilloma and laryngeal squamous cell carcinoma were 14.71% and 30.12%, respectively (χ2 = 3.006, P> 0.05). HPV18 In laryngeal papilloma tissue was not detected, and in laryngeal squamous cell carcinoma was 6.02%, the two groups, the difference was not significant (χ2 = 0.087, P> 0.05). The detection rate of HPV16 in highly differentiated laryngeal squamous cell carcinoma was significantly higher than that in moderately differentiated squamous cell carcinoma (χ2 = 6.032, P <0.05), while in well differentiated and poorly differentiated, moderately differentiated and poorly differentiated squamous cell carcinomas There was no significant difference between the two groups (χ2 = 0.351, 1.500, P> 0.05). There was no significant difference in the detection rate of HPV18 in different pathological differentiation laryngeal squamous cell carcinoma (χ2 = 0.060, P> 0.05). ④ Conclusion The occurrence of laryngeal squamous cell carcinoma is closely related to HPV infection, especially HPV16