论文部分内容阅读
目的:评价超滤工艺对注射用芪红脉通抗急性心肌缺血和被动皮肤过敏反应的影响。方法:选择舌静脉注射垂体后叶素(0.75 U·kg-1)构建急性心肌缺血大鼠模型,以心电图ST段变化和血清超氧化物歧化酶(SOD),丙二醛(MDA)的含量变化为药效学评价指标,比较注射用芪红脉通超滤前后对垂体后叶素致大鼠急性心肌缺血的影响,以大鼠被动皮肤过敏试验考察该制剂的安全性。结果:超滤前后注射用芪红脉通在成分上无明显变化;与模型组相比,超滤前后样品均能显著降低心电图ST段的抬升,超滤前中剂量及超滤后高剂量样品组均能显著提高血清SOD活性,各给药组均能显著降低血清MDA的产生,但超滤前后这些指标均无显著差异;超滤后样本的被动皮肤过敏呈阴性。结论:注射用芪红脉通具有显著的抗急性心肌缺血作用,超滤工艺提高了制剂的安全性,对药效无影响。
OBJECTIVE: To evaluate the effect of ultrafiltration on acute myocardial ischemia and passive cutaneous anaphylaxis induced by injection of QCHH. Methods: A rat model of acute myocardial ischemia was established by intravenous injection of vasopressin (0.75 U · kg -1). The changes of ST segment in ECG and the changes of serum superoxide dismutase (SOD), malondialdehyde (MDA) Content changes for the evaluation of pharmacodynamics, compared with before and after injection of veratridine ultrafiltration of pituitrin-induced acute myocardial ischemia in rat passive skin allergy test to investigate the safety of the preparation. Results: Compared with the model group, before and after ultrafiltration, the samples before and after ultrafiltration significantly decreased ST-segment elevation in the electrocardiogram, middle dose before ultrafiltration and high dose after ultrafiltration Group can significantly increase serum SOD activity, each treatment group can significantly reduce the production of serum MDA, but before and after ultrafiltration these indicators were no significant difference; passive skin allergy samples after the test was negative. Conclusion: QIHONGMIN injection has significant anti-acute myocardial ischemia, ultra-filtration technology to improve the safety of the preparation, no effect on the efficacy.