论文部分内容阅读
目的探讨 CCL4 诱导的肝纤维化模型鼠肝组织 Smad2/3,Smad7,TIMP-1,TGF-β1 的表达及氯沙坦干预后对其表达的影响。方法 Wistar大鼠 40 只,随机分成正常对照组、模型组、氯沙坦预防组和治疗组,采用 CCL4 皮下注射构建肝纤维化模型。行 HE 和 VG 染色,判断肝组织炎症和纤维化的程度。免疫组化方法检测各组 Smad2,3 Smad7 和 TIMP-1,TGF-β1 的表达。结果氯沙坦预防组和治疗组的肝组织炎症和纤维化程度明显低于模型组;Sm ad2,3 TIM P-1 TG F-β1 在氯沙坦预防组和治疗组的阳性表达均低于模型组( 分别为 Smad2,3:1.69 ±0.42,1.90 ±0.59,2.51 ±0.39;TIM P-1:1.09 ±0.28,1.32 ±0.23,2.60 ±0.35;TGF-β1:1.65±0.31,2.04±0.42,2.72±0.36),P <0.05;Sm ad7 在氯沙坦预防组和治疗组的阳性表达高于模型组( 分别为2.50±0.35,2.21±0.59,0.47±0.26),P <0.05。Smad2,3 Smad7 TIM P-1 和 TGF-β1 在氯沙坦预防组和治疗组的表达差异无显著性,P >0.05。结论氯沙坦抗肝纤维化作用可能与抑制 TGF-β1,TIMP-1,Smad2,3 和促进Smad7 的表达有关。
Objective To investigate the expression of Smad2 / 3, Smad7, TIMP-1 and TGF-β1 in hepatic tissue of CCL4-induced hepatic fibrosis model rats and the effect of losartan on the expression of Smad2 / 3, Methods Forty Wistar rats were randomly divided into normal control group, model group, losartan prevention group and treatment group. CCL4 subcutaneous injection was used to construct hepatic fibrosis model. HE and VG staining, to determine the degree of liver inflammation and fibrosis. Immunohistochemistry was used to detect the expression of Smad2, Smad7, TIMP-1 and TGF-β1 in each group. Results The levels of hepatic inflammation and fibrosis in losartan group and treatment group were significantly lower than those in model group. The positive expression of Sm ad2,3 TIMP-1 TG F-β1 in losartan group and treatment group were lower than The model group (Smad2,3: 1.69 ± 0.42,1.90 ± 0.59,2.51 ± 0.39; TIM P-1: 1.09 ± 0.28,1.32 ± 0.23,2.60 ± 0.35; TGF-β1: 1.65 ± 0.31,2.04 ± 0.42, 2.72 ± 0.36, P <0.05). The positive expression of Sm ad7 in Losartan prevention group and treatment group was higher than that in model group (2.50 ± 0.35,2.21 ± 0.59,0.47 ± 0.26, P <0.05). The expression of Smad2, Smad7 TIMP-1 and TGF-β1 in Losartan prevention group and treatment group had no significant difference (P> 0.05). Conclusion Losartan may play an important role in anti-hepatic fibrosis by inhibiting the expression of TGF-β1, TIMP-1, Smad2,3 and Smad7.