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在不同温度下,用荧光光谱、同步荧光光谱、三维荧光光谱和紫外可见吸收光谱法研究了茜素紫(Alizarin-Violet,ALV)与牛血清白蛋白(bovineserum albumin,BSA)相互作用特征。分别用Stern-Volmer方程、Lineweaver-Burk双倒数方程和热力学方程等处理试验数据,得到相互作用常数的平均值为1.078×105L/mol,相互作用的ΔHθ、ΔGθ和ΔSθ的平均值分别为-8.030kJ/mol、-29.19kJ/mol和69.80J/K,作用位置为2.38nm,作用位点数为1.173。证实了在试验浓度和温度范围内,ALV与BSA可相互作用形成具有一定结构的复合物,其荧光猝灭作用更符合静态猝灭作用特征,为研究ALV的生态环境效应、生物学效应和对蛋白质构象的影响以及ALV的染色机理等提供了重要信息。
The interaction between Alizarin-Violet (ALV) and bovine serum albumin (BSA) was studied by fluorescence, synchronous fluorescence, three-dimensional fluorescence spectroscopy and UV-Vis absorption spectroscopy at different temperatures. The experimental data of Stern-Volmer equation, Lineweaver-Burk double reciprocal equation and thermodynamic equation were used respectively to obtain the average value of the interaction constant of 1.078 × 105 L / mol. The average values of ΔHθ, ΔGθ and ΔSθ for interaction were -8.030 kJ / mol, -29.19kJ / mol and 69.80J / K, the site of action was 2.38nm, the number of sites for action was 1.173. It was confirmed that ALV interacts with BSA to form a complex with a certain structure at the concentration and temperature range of the experiment. The fluorescence quenching is more consistent with the static quenching characteristic. In order to study the ecological and biological effects of ALV and its biological effects, The conformation of the protein and the staining mechanism of ALV provide important information.