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目的:初步探讨伽玛刀治疗前后复发胶质母细胞瘤中P53及PCNA蛋白表达的变化及其临床意义。方法:用免疫组化SP法检测29例复发胶质母细胞瘤患者(伽玛刀刀治疗组12例,非伽玛刀治疗组17例)在初发和复发的肿瘤组织中P53蛋白和PCNA蛋白的表达。结果:两组患者在性别,年龄,肿瘤部位及大小构成上差异无统计学意义(P>0.05);两组的复发时间的差异有统计学意义(P=0.0409<0.05);在伽玛刀治疗组P53及PCNA蛋白在复发胶质母细胞瘤中表达明显降低(p53,t=3.915,P=0.02<0.05;PCNA,t=2.962,P=0.013<0.05);非伽玛刀治疗组p53及PCNA蛋白在复发胶质母细胞瘤中表达明显增加(p53,t=-5.926,P=0.000<0.05;PCNA,t=-5.160,P=0.000<0.05);P53及PCNA蛋白在伽玛刀治疗组和非伽玛刀治疗组的表达变化有统计学意义(p53,t=-5.577,P=0.000<0.05.PCNA,t=-5.542,P=0.000<0.05);在伽玛刀治疗组及非伽玛刀治疗组,P53蛋白和PCNA蛋白的阳性表达率不存在明显的相关性(伽玛刀治疗组,r=-0.085,P=0.792>0.05.非伽玛刀治疗组,r=0.450,P=0.07>0.05)。结论:P53及PCNA蛋白的异常表达与胶质母细胞瘤的复发有关,伽玛刀治疗胶质母细胞瘤瘤可能通过抑制P53及PCNA蛋白表达而起作用。
Objective: To investigate the changes of P53 and PCNA proteins in recurrent glioblastoma before and after Gamma Knife treatment and its clinical significance. Methods: Immunohistochemical SP method was used to detect the expression of P53 protein and PCNA in 29 patients with recurrent glioblastoma (12 in Gamma knife group and 17 in non-Gamma knife group) Protein expression. Results: There was no significant difference in gender, age, tumor location and size between the two groups (P> 0.05). The difference of recurrence time between the two groups was statistically significant (P = 0.0409 <0.05) The expression of P53 and PCNA in the treatment group was significantly lower than that in the recurrent glioblastoma (p53, t = 3.915, P = 0.02 <0.05; PCNA, t = 2.962, P = 0.013 <0.05) (P <0.05); PCNA, t = -5.160, P = 0.000 <0.05); P53 and PCNA protein in Gamma Knife Gamma Knife treatment group and non-Gamma knife treatment group, the expression of changes were statistically significant (P <0.05, P = 0.000 <0.05.PCNA, t = -5.542, P = 0.000 <0.05) There was no significant correlation between the expression of P53 protein and PCNA protein in non-gamma knife treatment group (Gamma Knife treatment group, r = -0.085, P = 0.792> 0.05) 0.450, P = 0.07> 0.05). Conclusion: The abnormal expression of P53 and PCNA proteins is related to the recurrence of glioblastoma. Gamma knife treatment of glioblastoma may play a role in inhibiting the expression of P53 and PCNA protein.