论文部分内容阅读
目的探讨P16和Ki-67在不同宫颈病变组织中的表达及临床意义。方法以127例宫颈上皮内瘤变(CIN)及宫颈癌(SCC)标本为研究组,30例正常宫颈组织及慢性宫颈炎标本作为对照组,采用免疫组织化学SP方法检测其组织中P16、Ki-67的表达水平。结果对照组组织中P16、Ki-67的表达水平显著低于CIN及SCC组,CINⅠ组P16、Ki-67的表达水平显著低于CINⅡ、CINⅢ及SCC组,差异均有统计学意义(P<0.05)。P16单独检测敏感度和特异度分别为65.35%、96.67%;Ki-67单独检测敏感度和特异度分别为69.29%、90.00%;两者联合检测敏感度和特异度分别为72.44%、90.00%。结论 P16、Ki-67的表达强度对宫颈良性改变的甄别、CIN级别的判定及宫颈早期浸润癌的诊断具有重要价值。
Objective To investigate the expression and clinical significance of P16 and Ki-67 in different cervical lesions. Methods 127 cases of cervical intraepithelial neoplasia (CIN) and cervical cancer (SCC) specimens of the study group, 30 cases of normal cervical tissue and chronic cervicitis specimens as a control group, the immunohistochemical SP method was used to detect the P16, Ki -67 expression levels. Results The expression of P16 and Ki-67 in the control group was significantly lower than that in the CIN and SCC groups. The expressions of P16 and Ki-67 in the CINⅠ group were significantly lower than those in the CINⅡ, CINⅢ and SCC groups (P < 0.05). The sensitivity and specificity of P16 alone were 65.35% and 96.67%, respectively. The sensitivity and specificity of Ki-67 alone were 69.29% and 90.00%, respectively. The sensitivity and specificity of combined detection of P16 and P16 were 72.44% and 90.00% . Conclusion The expression of P16 and Ki-67 is of great value in the screening of cervical benign changes, the determination of CIN grade and the diagnosis of early invasive carcinoma of the cervix.