论文部分内容阅读
目的探讨p16、Ki-67在慢性宫颈炎及宫颈鳞状上皮内病变中的表达及临床意义。方法收集本院宫颈活检及LEEP刀锥切标本共276例,其中慢性宫颈炎70例,LSIL68例(CINⅠ),HSIL138例(72例CINⅡ和66例CINⅢ)。采用免疫组化SP法检测p16及Ki-67表达情况。结果 p16在慢性宫颈炎、CINⅠ、CINⅡ和CINⅢ中的阳性率分别为5.7%、48.5%、80.6%和95.5%。p16阳性细胞与CIN级别一致的符合率分别为94.3%、89.7%、90.2%和95.5%。Ki-67≥50%在各组的阳性率分别为2.9%、89.7%、90.2%和95.5%。p16和Ki-67在慢性宫颈炎和CIN各组间的阳性率差异显著(P<0.05),p16阳性细胞与CIN级别的符合率差异不显著(P>0.05)。结论p16和Ki-67联合检测可有助于慢性宫颈炎及宫颈上皮内病变的诊断,可提高CIN分级的正确诊断率,有助于宫颈癌的早期筛查。
Objective To investigate the expression and clinical significance of p16 and Ki-67 in chronic cervicitis and cervical squamous intraepithelial lesion. Methods Totally 276 cases of cervix biopsy and LEEP knife conization were collected, including 70 cases of chronic cervicitis, 68 cases of LSIL (CINⅠ) and 138 cases of HSIL (72 cases of CINⅡ and 66 cases of CINⅢ). Immunohistochemical SP method was used to detect the expression of p16 and Ki-67. Results The positive rates of p16 in chronic cervicitis, CINⅠ, CINⅡ and CINⅢ were 5.7%, 48.5%, 80.6% and 95.5% respectively. The coincidence rates of p16 positive cells and CIN grade were 94.3%, 89.7%, 90.2% and 95.5% respectively. The positive rates of Ki-67≥50% in each group were 2.9%, 89.7%, 90.2% and 95.5% respectively. The positive rates of p16 and Ki-67 in patients with chronic cervicitis and CIN were significantly different (P <0.05). The coincidence rates of p16 and CIN in patients with chronic cervicitis and CIN were not significantly different (P> 0.05). Conclusion Combined detection of p16 and Ki-67 may be helpful for the diagnosis of chronic cervicitis and cervical intraepithelial lesion, which can improve the correct diagnosis rate of CIN classification and contribute to the early screening of cervical cancer.