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目的研究中国造血干细胞捐献者资料库甘肃分库汉族捐献者HLA-A、B、DRB1等位基因频率分布特征。方法采用反向特异性寡核苷酸探针杂交法(PCR-SSO)基因分型技术,对甘肃酒泉地区215份健康无血缘关系的汉族人群的人类白细胞抗原(HLA-A、-B、-DRB1)等位基因进行基因分型。结果在受检人群中共检出54种HLA特异性基因,A*02(32.47%)、A*11(20.62%)、A*24(14.95%)、B*15(15.72%)、B*40(13.4%)、DRB1*09(16.49%)、DRB1*12(15.46%)、DRB1*04(11.86%)和DRB1*15(10.57%)为本地区汉族人群的优势基因,其中A位点检出以往国内报道较少的A*74等位基因。结论甘肃酒泉汉族人群HLA-A、B、DRB1等位基因的分布特征接近北方汉族人群,与中国汉族人总体分布特征相似。未发现群体特有的基因,但具有其自身分布特征。这为不同地区、不同人群有可能在中国造血干细胞库甘肃地区汉族捐献者中寻找到HLA相合的供者提供了依据。
Objective To investigate the frequency distribution of HLA-A, B and DRB1 alleles in Han donors from the Gansu sub-database of Chinese hematopoietic stem cell donor database. Methods Reverse transcription-specific oligonucleotide probe hybridization (PCR-SSO) genotyping was used to detect the leukocyte antigen (HLA-A, -B, -) in 215 healthy unrelated Han Chinese population in Jiuquan district of Gansu province. DRB1) alleles. Results A total of 54 kinds of HLA-specific genes were detected in the subjects, including A * 02 (32.47%), A * 11 (20.62%), A * 24 (14.95%), B * 15 (13.4%), DRB1 * 09 (16.49%), DRB1 * 12 (15.46%), DRB1 * 04 (11.86%) and DRB1 * 15 Out of the past less reported A * 74 allele. Conclusion The distribution of HLA-A, B, DRB1 alleles in Han nationality in Jiuquan, Gansu Province is similar to that of Han population in northern China, which is similar to the overall distribution characteristics of Chinese Han population. No group-specific genes were found but had their own distributional characteristics. This provided a basis for finding HLA-matched donors among Chinese donors in the Chinese hematopoietic stem cell-based Kansu region in different regions and different populations.