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目的 :探讨T淋巴细胞核仁区酸性非组蛋白表达活性 (Ag -NORs)对肾移植受者术前及术后用药病情监测的应用价值。方法 :应用普通细胞银染技术对 32例肾移植患者术前及术后使用免疫抑制剂 1,2 ,3周外周血T淋巴细胞染色 ,通过计算机显微图像分析技术 ,计算硝酸银染色酸性非组蛋白 (Ag -NORs)面积与核面积的比值 (I·S % )。结果 :肾移植患者术前Ag -NORs的I·S %为 (6 .31± 0 .86 ) ;术后使用免疫抑制剂 1,2 ,3周Ag -NORs的I·S %分别为 (4.72± 0 .93) ,(4.2 8± 1.0 5 ) ,(4.6 6± 1.2 1)。肾移植术后各期与术前Ag -NORs的I·S %差异有显著性意义 (P <0 .0 1) ;术后各期之间Ag -NORs的I·S %差异无显著性意义 (P >0 .0 5 )。结论 :T淋巴细胞核仁酸性非组蛋白表达活性在肾移植及其术后用药病情监测中具有重要的意义。
Objective: To investigate the value of Ag-NOR in nucleolar zone of T lymphocytes in the monitoring of preoperative and postoperative drug use in renal transplant recipients. Methods: Thirty-two cases of renal allograft recipients were immunostained with T-lymphocytes of 1, 2 and 3 weeks before and after operation by silver staining technique. The cytotoxicity of silver nitrate staining was calculated by computer microscopic image analysis The ratio of Ag-NORs area to core area (I · S%). Results: The I · S% of preoperative Ag-NORs in renal transplant patients was (6.31 ± 0.86). The I · S% of Ag-NORs at 1, 2, and 3 weeks after immunosuppression were (4.72 ± 0.93), (4.2 8 ± 1.0 5), (4.6 6 ± 1.2 1). There was a significant difference in I · S% of Ag-NORs between preoperative and postoperative renal transplantation (P <0.01). There was no significant difference in I · S% of Ag-NORs between postoperative stages (P> 0.05). CONCLUSION: The activity of nucleolar acidic non-histone protein expression in T lymphocytes plays an important role in the monitoring of kidney transplantation and postoperative drug use.