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目的:探讨PTEN蛋白基因及微血管密度(MVD)表达对垂体腺瘤侵袭性的影响。方法:随机抽取我院神经外科200例存档的垂体腺瘤手术标本,其中侵袭性垂体腺瘤(侵袭组)72例,非侵袭性垂体腺瘤(非侵袭组)136例。采用免疫组化SP法对两组标本进行PTEN蛋白基因和MVD检测,并对两组表达强度进行相关分析。结果:两组垂体腺瘤组织中均可见PTEN蛋白基因染色,主要位于胞质内。其中侵袭组PTEN蛋白基因阳性率为38.8%,非侵袭组为82.4%;两组比较差异非常显著(P<0.01)。两组MVD染色均有表达,其中侵袭组MVD表达为(16.82±6.72)%,非侵袭组为(9.25±4.86)%;两组比较差异非常显著(P<0.01)。侵袭组PTEN蛋白基因染色强阳性为1例(1.4%),非侵袭组为15例(11.0%);两组比较,差异非常显著(P<0.01)。相关分析显示,PTEN与MVD间呈非常显著负相关(P<0.01)。结论:PTEN蛋白基因突变和(或)缺失与血管生成可能是垂体腺瘤向侵袭性转变的重要影响因素,PTEN蛋白基因的低表达可能在垂体肿瘤血管生成过程中发挥一定作用。
Objective: To investigate the effect of PTEN protein gene and microvessel density (MVD) on the invasiveness of pituitary adenomas. Methods: Two hundred surgical specimens of pituitary adenomas were collected randomly from our department of neurosurgery, including 72 cases of invasive pituitary adenoma (invasion group) and 136 cases of non-invasive pituitary adenoma (non-invasive group). The immunohistochemical SP method was used to detect the PTEN protein gene and MVD in the two groups of specimens, and the correlation analysis was made between the two groups. Results: PTEN protein staining was found in both groups of pituitary adenomas, mainly located in the cytoplasm. The positive rate of PTEN protein was 38.8% in invasive group and 82.4% in non-invasive group. The difference between the two groups was significant (P <0.01). The expression of MVD in both groups was significant (16.82 ± 6.72%) in invasive group and (9.25 ± 4.86)% in non-invasive group. The difference between the two groups was significant (P <0.01). In the invasive group, PTEN protein gene staining was strongly positive in 1 case (1.4%) and in non-invasive group (15 cases, 11.0%). The difference between the two groups was significant (P <0.01). Correlation analysis showed that there was a significant negative correlation between PTEN and MVD (P <0.01). CONCLUSION: Mutations and / or deletions of PTEN protein and angiogenesis may play an important role in invasive invasion of pituitary adenomas. The low expression of PTEN protein may play a role in the angiogenesis of pituitary tumors.