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本文研究小白鼠感染鼠疟原虫(Plasmo-dium berghei yoelii)后的组织变化,特别是脾脏的胸腺依赖区,探索T和B淋巴细胞间的免疫作用问题。小白鼠系3～6月龄的纯种雌鼠,先以子孢子感染后,取血注射腹腔感染实验鼠,在不同天后杀死。组织学检查用Carnoy氏液固定,以苏木素—伊红和派罗宁甲基绿染色(用Gomori氏法染脾脏网织纤维)。组织的免疫萤光染色是将快速冰冻(-70℃)切片,经丙酮固定后,用免抗鼠免疫球蛋白(γG1,γG2a,γG2bγM)及β_1C球
In this paper, we investigated the tissue changes in mice infected with Plasmodium dioetille, in particular the thymus-dependent region of the spleen, and explored the immunological effects between T and B lymphocytes. Department of white mice 3 to 6 months old purebred females, the first sporozoite infection, blood infection intraperitoneally infected mice, killed in different days. Histological examination was fixed with Carnoy’s solution and stained with hematoxylin-eosin and palonine methyl green stain (spleen reticulated fibers by Gomori’s method). Tissue immunofluorescence staining was rapid freezing (-70 ℃) section, fixed with acetone, with anti-mouse immunoglobulin (γG1, γG2a, γG2bγM) and β_1C ball