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目的:探讨吡格列酮对大鼠碱烧伤后角膜血管内皮细胞生长因子(VEGF),碱性成纤维细胞生长因子(b-FGF)表达的调节作用,以及对碱烧伤后角膜新生血管生长的抑制作用。方法:建立大鼠角膜碱烧伤模型,结膜下注射吡格列酮,观察烧伤后不同时期的角膜新生血管的数量、长度,应用免疫组化和Western blot方法检测碱烧伤后及烧伤治疗不同时期的VEGF,b-FGF的表达变化情况,并与对照组比较。结果:大鼠角膜碱烧伤后2d角膜新生血管开始生长,7～10d达到高峰,吡格列酮治疗后的角膜新生血管的发生延迟,血管的生长受到抑制,VEGF,b-FGF随时间发生变化,表达较对照组下降。结论:局部应用吡格列酮对由碱烧伤引起的鼠角膜新生血管的发生、发展有明显的抑制作用,其机理可能是通过下调促血管生成因子VEGF,b-FGF的表达而实现的。
AIM: To investigate the regulatory effect of pioglitazone on the expression of corneal vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) after alkali burn in rats and the inhibition of corneal neovascularization after alkali burn. Methods: The model of corneal alkali burn in rats was established. Pioglitazone was injected subconjunctivally. The number and length of corneal neovascularization were observed at different stages after burns. The expressions of VEGF and b were detected by immunohistochemistry and Western blot in different periods after burn treatment -FGF expression changes, and compared with the control group. Results: Corneal neovascularization began to grow 2 days after corneal alkali burn in rats and peaked at 7 to 10 days. Corneal neovascularization was delayed after pioglitazone treatment. The growth of blood vessels was inhibited. The expression of VEGF and b-FGF was changed with time Control group decreased. CONCLUSION: Topical application of pioglitazone significantly inhibits the occurrence and development of rat corneal neovascularization induced by alkaline burn. The mechanism may be through down-regulating the expression of pro-angiogenic factors VEGF and b-FGF.