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目的探讨多西紫杉醇对鼠血管肉瘤细胞ISOS-1凋亡和增殖的影响。方法建立ISOS-1鼠血管肉瘤动物模型,20只移植瘤模型小鼠分为4组,每组5只,即①5mg/kg多西紫杉醇组;②10mg/kg多西紫杉醇组;③20mg/kg多西紫杉醇组;④对照组。静脉给予多西紫杉醇,每周1次。测定肿瘤体积,观察超微结构改变,并采用脱氧核苷酸末端转移酶介导的缺口末端标记法检测组织细胞凋亡情况。结果多西紫杉醇剂量为5mg/kg、10mg/kg、20mg/kg时,其瘤体积抑制率分别为71%、77%、79%,与对照组比较,可见到有意义的肿瘤增殖抑制效果(P<0.01);多西紫杉醇各剂量组超微结构观察,可见早期凋亡改变,ISOS-1细胞凋亡率均显著高于对照组(P<0.01)。结论多西紫杉醇具有诱导鼠血管肉瘤细胞ISOS-1凋亡和抑制其在小鼠体内增殖的作用。
Objective To investigate the effect of docetaxel on the apoptosis and proliferation of murine angiosarcoma cells ISOS-1. Methods The animal model of ISOS-1 mouse angiosarcoma was established. Twenty tumor models were divided into 4 groups (5 mg / kg docetaxel group), 10 mg / kg docetaxel group (20 mg / kg docetaxel group) Paclitaxel group; ④ control group. Intravenous docetaxel, once a week. Tumor volume was measured to observe ultrastructural changes, and the apoptosis of tissue cells was detected by the nick end labeling method mediated by deoxynucleotidyl transferase. Results The tumor volume inhibition rates of docetaxel at 5 mg / kg, 10 mg / kg and 20 mg / kg were 71%, 77% and 79%, respectively. Compared with the control group, P <0.01). The ultrastructural changes in docetaxel groups showed that the apoptosis rate of ISOS-1 cells was significantly higher than that of the control group (P <0.01). Conclusion Docetaxel can induce the apoptosis of murine angiosarcoma cell line ISOS-1 and inhibit its proliferation in mice.