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目的:观察升清降浊胶囊对高糖诱导的腹膜纤维化大鼠分泌高迁移率族蛋白1(HMGB)1和转化生长因子β1(TGF-β)1的影响。方法:将SD雄性大鼠40只随机分成4组:空白组、模型组、低剂量组、高剂量组各10只。空白组与模型组予5mL生理盐水灌胃,低剂量组予升清降浊胶囊50mg/(kg.d)、高剂量组予升清降浊胶囊100mg/(kg.d),由生理盐水稀释至5mL后灌胃,每天1次,共4周。分别于实验第3、7、14、28天时大鼠尾静脉取血检测HMGB1和TGF-β1,第28天处死各组大鼠,留取壁层腹膜组织做HE及Masson染色,测量腹膜厚度。结果:模型组HMGB1及TGF-β1的含量、腹膜致密层厚度均高于空白组(P<0.05);中药低剂量组HMGB1及TGF-β1含量、腹膜致密层厚度均低于模型组(P<0.05);中药高剂量组HMGB1及TGF-β1含量、腹膜致密层厚度降低更明显,与低剂量组比较,差异均有显著性意义(P<0.05)。结论:升清降浊胶囊可以降低HMGB1、TGF-β1含量,降低腹膜致密层厚度,且随着剂量增加效果更明显,提示升清降浊胶囊对延缓腹膜纤维化有一定意义。
Objective: To observe the effects of Shengqing Jiangzhuo Capsule on high mobility group box 1 (HMGB1) and transforming growth factor β1 (TGF-β1) secreted by high glucose-induced peritoneal fibrosis in rats. Methods: Forty SD male rats were randomly divided into 4 groups: blank group, model group, low dose group and high dose group. The rats in the blank group and model group were intragastrically administered with 5 mL saline, the low-dose group was treated with Shengqing Jiangzhuo capsule 50mg / (kg · d), the high-dose Shengjing Qingzhuo capsule 100mg / (kg.d) After 5mL gavage, 1 day, a total of 4 weeks. The rats were sacrificed on days 3, 7, 14 and 28 for HMGB1 and TGF-β1 in the tail vein respectively. The rats in each group were killed on the 28th day. The peritoneum in the parietal layer was taken for HE and Masson staining, and the peritoneal thickness was measured. Results: The content of HMGB1 and TGF-β1 and the density of peritoneal dense layer in model group were higher than those in blank group (P <0.05). The content of HMGB1, TGF-β1 and the density of peritoneal dense layer in model group were lower than those in model group (P < 0.05). The levels of HMGB1 and TGF-β1 in the high-dose group of Chinese medicine were significantly lower than those in the low-dose group (P <0.05). Conclusion: Shengqing Jiangzhuo Capsule can reduce the contents of HMGB1 and TGF-β1 and decrease the density of peritoneal dense layer. It is more obvious with increasing dose, which suggests that Shengqing Jiangzhuo Capsule has a certain significance in delaying peritoneal fibrosis.