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目的:探讨P120和E-cad在乳腺浸润性导管癌与浸润性小叶癌中的表达及其鉴别诊断意义。方法:收集海口市人民医院2005年1月～2010年12月间有完整随访资料的浸润性导管癌32例,浸润性小叶癌16例及混合性癌12例。采用SP免疫组化染色检测P120连环蛋白、E-钙黏蛋白和P63抑癌基因表达情况。结果:P120连环蛋白在32例浸润性导管癌中胞膜阳性率为100.0%;在16例浸润性小叶癌中胞浆阳性率为100.0%。E-钙黏蛋白在浸润性导管癌中胞膜阳性率为84.3%(27/32),而在小叶癌中无阳性表达(P<0.01)。P63在浸润性导管癌中有1例阳性其余均为阴性;在小叶癌中3例阳性其余为阴性,二者差异无统计学意义(P>0.05)。P120和E-钙黏蛋白联合应用将12例混合性癌分为浸润性导管癌8例,浸润性小叶癌4例。结论:P120和E-钙黏蛋白联合应用可区分浸润性导管癌和小叶癌,而P63则不能用于二者的鉴别。
Objective: To investigate the expression of P120 and E-cad in invasive ductal carcinoma of the breast and invasive lobular carcinoma and their differential diagnostic significance. Methods: 32 cases of infiltrating ductal carcinoma, 16 cases of invasive lobular carcinoma and 12 cases of mixed carcinoma with complete follow-up data were collected from Haikou People’s Hospital from January 2005 to December 2010. SP immunohistochemical staining was used to detect the expression of P120 catenin, E-cadherin and P63 tumor suppressor gene. Results: The positive rate of P120 catenin in 32 invasive ductal carcinomas was 100.0%. The positive rate of cytoplasm was 100.0% in 16 cases of invasive lobular carcinoma. The positive rate of E-cadherin in invasive ductal carcinoma was 84.3% (27/32), but not in lobular carcinoma (P <0.01). P63 in invasive ductal carcinoma in 1 case of the remaining were negative; in lobular carcinoma in 3 cases the rest was negative, the difference was not statistically significant (P> 0.05). In combination with P120 and E-cadherin, 12 cases of mixed carcinomas were divided into invasive ductal carcinoma in 8 cases and invasive lobular carcinoma in 4 cases. Conclusion: The combination of P120 and E-cadherin can differentiate invasive ductal carcinoma from lobular carcinoma, while P63 can not be used for the identification of the two.