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为筛选北京油鸡和来航鸡脾脏组织中与抗病力差异相关的候选基因和信号通路,本研究采用高通量测序技术,构建了42日龄北京油鸡(抗病力较强)和来航鸡(抗病力相对较弱)脾脏组织的数字基因表达谱,对差异表达基因进行了GO功能分类、KEGG信号通路分析和STRING互作网络构建,并利用荧光定量PCR验证了部分差异表达基因。结果表明,以来航鸡为对照组,北京油鸡与之相比,差异倍数在2倍以上的共有1335个基因,其中693个上调表达,643个下调表达,包括多个与淋巴细胞的激活、分化和增殖、免疫器官发育等相关的基因,主要涉及到结合功能、细胞组成、细胞加工和生物学调节等功能。对通路显著性分析发现,与免疫相关生物学通路共有4个,其中BCR信号和TLR信号涉及脾脏B细胞介导的体液免疫应答反应,两条通路上的差异表达基因构成1个相互连接的互作网络,其中D79B、CD44、IL1B、SOCS1及TLR4等位于重要节点位置。为今后挖掘新基因和研究影响鸡抗病力的遗传因素等提供理论依据。
In order to screen candidate genes and signal pathways related to disease resistance in spleen tissues of Beijing Youji and Laihang chicken, this study used high-throughput sequencing technology to construct Beijing 42-day-old Beijing Youji (strong resistance) The gene expression profile of spleen in chickens (relatively weaker disease resistance) was divided into GO functional classification, KEGG signaling pathway analysis and STRING interaction network. Some differentially expressed genes were identified by fluorescence quantitative PCR. The results show that since the chicken as the control group, Beijing oil chicken compared with a multiple of more than 2 times the total of 1335 genes, of which 693 up-regulated, 643 down-regulated, including a number of lymphocyte activation, Differentiation and proliferation, immune organ development and other related genes, mainly related to the binding function, cell composition, cell processing and biological regulation and other functions. Significant analysis of the pathway found that immune-related biological pathways a total of four, including BCR signal and TLR signal involved in spleen B cell-mediated humoral immune response, the two pathways of the differentially expressed genes constitute a mutually connected As a network, D79B, CD44, IL1B, SOCS1 and TLR4 are located at important nodes. Provide a theoretical basis for digging new genes in the future and studying the genetic factors affecting the disease resistance of chickens.