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目的：克隆和表达６Ｂ１１卵巢癌抗独特型抗体单链可变区基因，并进行序列分析。方法：采用逆转录ＰＣＲ和基因重组技术，扩增并构建６Ｂ１１鼠抗独特型抗体单链可变区基因，重组至噬菌体表面表达载体，在大肠杆菌表达，并利用双脱氧末端终止法进行核苷酸序列分析。结果：阳性克隆表达产物与卵巢癌单克隆抗体ＣＯＣ１６６－９特异结合；ＤＮＡ序列分析表明重链和轻链可变区分属小鼠免疫球蛋白重链第Ｉ亚群和轻链第Ⅱ亚群。结论：自６Ｂ１１小鼠杂交瘤细胞成功地克隆表达了该抗独特型单链抗体基因，为进一步人源化改造，推进卵巢癌的免疫治疗奠定了良好基础。
OBJECTIVE: To clone and express the single chain variable region gene of 6B11 ovarian cancer anti-idiotypic antibody and to analyze the sequence. Methods: The single-chain variable region of 6B11 murine anti-idiotypic antibody was amplified by reverse transcription-polymerase chain reaction (PCR) and gene recombination. The gene was expressed in E. coli and expressed in E.coli. The dideoxy-terminal deoxynucleotidyl transferase Acid sequence analysis. Results: The positive clones showed specific binding to COC166-9, a monoclonal antibody against ovarian cancer. DNA sequence analysis showed that the variable regions of heavy chain and light chain were mouse immunoglobulin heavy chain subgroup I and light chain subgroup Ⅱ. CONCLUSION: The anti-idiotypic single chain antibody gene was successfully cloned and expressed from 6B11 mouse hybridoma cells, which laid a good foundation for further humanized transformation and promotion of immunotherapy for ovarian cancer.