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本实验将含有5-羟色胺(5-HT)和多巴胺(DA)能性神经元的胎兔脑组织微块悬液用立体定向法移植至成年家兔脑内。21只兔分为三组,每组7只:Ⅰ组加入神经生长因子(NGF),Ⅱ组加入成纤维细胞生长因子(FGF),Ⅲ组未加任何因子列为对照组。移植2个月后用高效液相色谱电化学法(HPLC-EC)测定移植神经元递质(5-HT、DA)含量。结果显示Ⅰ、Ⅱ两组的5-HT 和 DA 含量均显著高于对照组(P<0.05,P<0.01,除Ⅰ组 DA 含量外),从而表明 NGF 和 FGF 可能具有促进移植神经元存活、成熟和递质释放的功能。
In this experiment, fetal brain tissue microtiter suspension containing serotonin (5-HT) and dopamine (DA) neurons was transplanted into adult rabbit brain by stereotactic method. Twenty-one rabbits were divided into three groups with 7 mice in each group: group I was given NGF, group II was added fibroblast growth factor (FGF), and group III was added as control. Two months after transplantation, the contents of neurotransmitter (5-HT, DA) were measured by HPLC-EC. The results showed that the contents of 5-HT and DA in group Ⅰ and group Ⅱ were significantly higher than that in control group (P <0.05, P <0.01, except for the content of DA in group Ⅰ), which indicated that NGF and FGF may promote the survival of transplanted neurons, Mature and neurotransmitter function.