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目的联合采用培养、电镜观察及ＰＣＲ检验技术，对沪、宁两地部分高危人群的泌尿生殖道分泌物标本进行分离及鉴定。方法沪宁受检者７１例，宫颈或尿道口内拭子分泌物接种于５ｍｌＳＰ－４培养基３７℃培养。对初代分离的阳性株用ＰＣＲ技术进行扩增以鉴定ＭＧＤＮＡ。对阳性株再行电镜负染观察其形态特征。结果７１份标本中培养分离出２株ＭＧ，分离率为２．８２％。分离培养的阳性株满足ＭＧ生长代谢的基本特性，ＰＣＲ在标本及培养物中均可扩增出特异性序列片段；电镜下呈现ＭＧ特征性外观，可呈烧瓶状、鸭梨状或纺锤状。结论采用培养方法可在高危人群中分离ＭＧ感染株，其分离株具生长时间长，需特制培基和特殊生化反应性能，具特征性外观形态等。
Objective To isolate and identify genitourinary secretions from some high-risk groups in Shanghai and Ningxia by culture, electron microscopy and PCR. Methods 71 cases of Shanghai-Nanjing subjects, cervical or urethral swab secretions were inoculated in 5mlSP-4 medium 37 ℃ culture. The positive isolates from the first generation were amplified by PCR to identify MG-DNA. The morphological characteristics of positive strains were observed by negative electron microscopy. Results Two strains of MG were isolated from 71 samples and the isolation rate was 2.82%. The positive strains isolated and cultured can meet the basic characteristics of MG growth and metabolism. PCR can amplify the specific sequence fragments in the specimens and cultures. The characteristic appearance of MG appears under the electron microscope and can be flasks, pear-shaped or spindle-shaped. Conclusion The culture method can be used to isolate MG-infected strains in high-risk groups. The isolates have long-term growth, special culture medium and special biochemical reactions, with characteristic appearance and morphology.