论文部分内容阅读
目的探讨先天性心脏病患儿不同灌注方法体外循环(CPB)下术后外周血IL-6、10的变化及其意义。方法选择先天性心脏病患儿40例。随机分为晶体停搏液灌注组(A组)和含血停搏液灌注组(B组),每组各20例。采用ELISA测定二组患儿麻醉诱导前、CPB结束后不同时间点外周血血清IL-6、10水平。应用SPSS13.0软件进行统计学分析。结果A组术前外周血IL-6、10水平与B组比较无显著性差异,术后二组患儿血清IL-6、10水平较术前均有不同程度升高(Pa<0.05),术后各时间点B组IL-6、10水平与A组比较均有显著性差异(Pa<0.05)。患儿外周血IL-6与IL-10水平呈正相关(r=10.32P<0.05)。结论CPB心脏术后IL-6、10显著升高,灌注含血停搏液可明显降低IL-6、10水平,减轻全身炎性反应、心肌损伤,与灌注晶体停搏液比较,含血停搏液有更好的心肌保护作用。
Objective To investigate the changes and significance of IL-6 and 10 in peripheral blood after cardiopulmonary bypass (CPB) with different perfusion methods in children with congenital heart disease. Methods Forty children with congenital heart disease were selected. Randomly divided into crystalloid cardioplegia perfusion group (A group) and blood cardioplegia perfusion group (B group), each group of 20 cases. Serum levels of IL-6 and 10 were measured by ELISA before anesthesia induction and at different time points after CPB in both groups. Application SPSS13.0 software for statistical analysis. Results The level of IL-6 and 10 in preoperative peripheral blood in group A was not significantly different from that in group B, and the levels of IL-6 and 10 in both groups were significantly higher than those before operation (Pa0.05) The levels of IL-6 and 10 in group B after operation were significantly different from those in group A (P <0.05). There was a positive correlation between IL-6 and IL-10 in children with peripheral blood (r = 10.32 P <0.05). Conclusions After CPB, the levels of IL-6 and 10 are significantly increased. Perfusion of blood cardioplegia can significantly reduce the level of IL-6 and 10, relieve the systemic inflammatory reaction and myocardial injury. Compared with perfusion crystalloid cardioplegia, Beating fluid has better myocardial protection.