OsRRMh, a Spen-Like Gene, Plays an Important Role During the Vegetative to Reproductive Transition i

来源 :Journal of Integrative Plant Biology | 被引量 : 0次 | 上传用户:w7622420
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OsRRMh,a homologue of OsRRM,encodes a Spen‐like protein,and is composed of two N‐terminal RNA recognition motifs(RRM)and one C‐terminal Spen paralogue and an orthologue C‐terminal domain(SPOC).The gene has been found to be constitutively expressed in the root,stem,leaf,spikelet,and immature seed,and alternative splicing patterns were confrmed in different tissues,which may indicate diverse functions for OsRRMh.The OsRRMh dsRNAi lines exhibited late‐fowering and a larger panicle phenotype.When full‐length OsRRMh and/or its SPOC domain were overexpressed,the fertility rate and number of spikelets per panicle were both markedly reduced.Also,overexpression of OsRRMh in the Arabidopsis fpa mutant did not restore the normal fowering time,and it delayed fowering in Col plants.Therefore,we propose that OsRRMh may confer one of its functions in the vegetative‐to‐reproductive transition in rice(Oryza sativa L.subsp.japonica cv.Zhonghua No.11(ZH11)). OsRRMh, a homologue of OsRRM, encodes a Spen-like protein, and is composed of two N-terminal RNA recognition motifs (RRM) and one C-terminal Spen paralogue and an orthologue C-terminal domain found to be constitutively expressed in the root, stem, leaf, spikelet, and immature seed, and alternative splicing patterns were confrmed in different tissues, which may indicate different functions for OsRRMh.The OsRRMh dsRNAi lines exhibited late-fowering and a larger panicle phenotype . Full-length OsRRMh and / or its SPOC domain were overexpressed, the fertility rate and number of spikelets per panicle were both markedly reduced. Also, overexpression of OsRRMh in the Arabidopsis fpa mutant did not restore the normal fowering time, and it delayed fowering in Col plants. Beforefore, we propose that OsRRMh may confer one of its functions in the vegetative-to-reproductive transition in rice (Oryza sativa L. subsp. japonica cv. Zhonghua No.11 (ZH11)).
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