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目的:研究阐明北京地区人冠状病毒(human coronavirus，HCoV)的流行特征和基因变异规律，为HCoV的感染防控提供分子流行病学依据。方法:本研究收集了2016—2017年北京市呼吸道多病原监测采集的HCoV阳性病例样本287份和流行病学数据，分析HCoV检出率、人群、季节特征，采用RT-PCR方法扩增HCoV pol基因部分片段用于HCoV分型和进化分析研究。结果:本研究HCoV检出率为1.89%，HCoV阳性样本的呼吸道病毒共感染率为20.56%(59/287)。鉴定出OC43型69株，核苷酸同源性为97.2%～100%，氨基酸同源性为92.2%～100%；229E型39株，核苷酸同源性为99.2%～100%，氨基酸同源性为99.2%～100%；HKU1型10株，NL63型2株。结论:OC43型和229E型为2016—2017年北京市HCoV主要型别，呈现出一定的季节差异，同一型别流行株具有很高的核苷酸和氨基酸同源性。“,”Objective:To study the prevalence and gene variation characteristics of human coronavirus (HCoV) in Beijing and provide molecular epidemiological basis for the prevention and control of HCoV infection.Methods:This study collected 287 samples and epidemiological data of HCoV positive cases collected by respiratory multi-pathogen surveillance in Beijing from 2016 to 2017, and analyzed the HCoV detection rate, population and seasonal characteristics. HCoV pol gene fragments were amplified by RT-PCR for HCoV typing and evolutionary analysis.Results:In this study, the detection rate of HCoV was 1.89%. The co-infection rate of respiratory virus in HCoV positive samples was 20.56% (59/287). 69 strains were identified as type OC43 with nucleotide homology of 97.2%~100% and amino acid homology of 92.2%~100%. Thirty-nine strains were identified as type 229E with nucleotide homology of 99.2%~100% and amino acid homology of 99.2%~100%. Ten strains were identified as type HKU1 and 2 strains were identified as type NL63.Conclusions:OC43 and 229E are still the main types of HCoV in Beijing from 2016 to 2017, and both present certain seasonal difference. The endemic strains of the same type have high homology of nucleotide and amino acid.