论文部分内容阅读
目的 研究艾氏腹水瘤全细胞瘤苗诱导的特异性杀伤活性 .方法 用 40 g·L- 1 的多聚甲醛处理的艾氏腹水瘤细胞作为全细胞瘤苗免疫 BAL B/ c小鼠 ,建立瘤苗小鼠模型 .用 HE染色法对亲本肿瘤细胞皮下再次攻击形成的肿瘤结节进行病理学观察 ;用 5 1 Cr释放法测定瘤苗免疫组、荷瘤组、正常组小鼠脾细胞 ,对亲本艾氏腹水瘤细胞和 Sp2 / 0细胞的杀伤活性 .结果 HE染色显示 ,经瘤苗免疫后亲本肿瘤细胞皮下再次攻击形成的肿瘤结节中 ,瘤细胞几乎完全坏死 ,同时在坏死部位有大量炎细胞浸润、纤维母细胞和小血管增生 ;而对照组则无以上现象 .效靶比为 2 0 0∶ 1时 ,免疫小鼠脾细胞体外杀伤亲本艾氏腹水瘤细胞的杀伤率 (% )为 (4 2 .3± 3.2 ) % ,显著高于荷瘤组的 (12 .1± 2 .3) %、正常组的 (6 .1± 1.1) %和对 Sp2 / 0细胞的杀伤率 (8.8±0 .4) % (P均 <0 .0 5 ) .结论 艾氏腹水瘤全细胞瘤苗可诱导机体产生特异性杀伤活性 .
Objective To study the specific killing activity of whole cell vaccine induced by Ehrlich ascites. Methods Ehrlich ascites tumor cells treated with 40 g·L -1 paraformaldehyde were used as whole cell vaccine to immunize BAL B/c mice. In the mouse model of tumor vaccine, the tumor nodules formed by subcutaneous re-challenge of parental tumor cells were observed by HE staining. The spleen cells of mice immunized group, tumor-bearing group and normal group were determined by 51 Cr release method. The killing activity of parental Ehrlich ascites tumor cells and Sp2 / 0 cells. Results HE staining showed that after tumor seedlings immunized with parental tumor cells subcutaneously re-attacked tumor nodules, the tumor cells were almost completely necrotic, and at the same time there were necrotic sites. A large number of inflammatory cell infiltrates, fibroblasts, and small blood vessel hyperplasia occurred; in the control group, there was no such phenomenon. When the effect-to-target ratio was 2:0:1, the spleen cells of the immunized mice killed the parental Ehrlich ascites tumor cells in vitro. ) was (42.3 ± 3.2)%, significantly higher than (12.1 ± 2.3)% in tumor-bearing group, (6.1 ± 1.1)% in normal group, and killing rate on Sp2 / 0 cells. (8.8 ± 0.4) % (P all <0.05). Conclusion Ehrlich ascites tumor Cell vaccine can induce specific cytotoxic activity.