目的研究艾氏腹水瘤全细胞瘤苗诱导的特异性杀伤活性。方法用40g/L的多聚甲醛处理的艾氏腹水瘤细胞作为全细胞瘤苗免疫Balb/c小鼠,建立瘤苗小鼠模型。用HE染色法对亲本肿瘤细胞皮下再次攻击形成的肿瘤结节进行病理学观察;用51Cr释放法测定瘤苗免疫组、荷瘤组、正常组小鼠脾细胞,对亲本艾氏腹水瘤细胞和Sp2细胞的杀伤活性。结果HE染色显示,经瘤苗免疫后亲本肿瘤细胞皮下再次攻击形成的肿瘤结节中,瘤细胞几乎完全坏死,同时在坏死部位有大量炎细胞浸润、纤维母细胞和小血管增生而对照组则无以上现象。效靶比为200∶1时,免疫小鼠脾细胞体外杀伤亲本艾氏腹水瘤细胞的杀伤率%为42.3±3.2%,显著高于荷瘤组的12.1±2.3%、正常组的6.1±1.1%和对Sp2/0细胞的杀伤率8.8±0.4%P均0.05。结论艾氏腹水瘤全细胞瘤苗可诱导机体产生特异性杀伤活性。 Objective To study the specific killing activity induced by Ehrlich ascites tumor cell vaccine. Methods Balb / c mice were immunized with 40g / L paraformaldehyde Ehrlich ascites tumor cells as a whole cell vaccine to establish a tumor-bearing mouse model. The tumor nodules formed by subcutaneous re-attack of the parental tumor cells were stained with hematoxylin-eosin (HE) staining. Pathological observation was made on the tumor nodules formed by the subcutaneous re-attack of the parental tumor cells. The 51Cr release assay was used to determine the spleen cells of the vaccine-immunized group, Sp2 cells. Results Hematoxylin-eosin staining showed that tumor cells were almost completely necrotic in the tumor nodules which were subcutaneously challenged by the parental tumor cells after tumor vaccine immunization. At the same time, there were a large number of infiltration of inflammatory cells, fibroblasts and small blood vessels in the necrotic sites, No above phenomenon. The killing rate of Ehrlich ascites cells in vitro was 42.3 ± 3.2%, which was significantly higher than that of the tumor-bearing group (12.1 ± 2.3%) and that of the normal group (6.1 ± 1.1) % And killing rate of Sp2 / 0 cells 8.8 ± 0.4% P 0.05. Conclusion Ehrlich ascites tumor cell vaccine can induce specific killing activity.