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本实验采用 Na~+、K~+及参比三管离子选择性微电极(Ion—Selective Microelectrodes,ISMs)同时动态检测14只在体 Wistar 大白鼠正常脑皮层及大脑中动脉凝闭(Middle cerebralartery occlusion,MCAO)后脑梗塞区细胞外 Na~+及 K~+活度的动态变化以及对 Na~+、K~+及参比三管 ISMs 的性能进行测试。实验结果显示:MCAO 后1—2分钟脑梗塞区细胞外 Na~+迅速从正常平均值140.2±9.3mM(±SD)下降到129.7±10.2mM(±SD)。而 K~+则从正常的平均值4.29±0.41mM(±SD)升高到6.12±1.00mM(±SD)。Na~+电极的线性范围在10~(-5)—10~(-1)M,K~+电极的线性范围在10~(-1)—10~(-6)M,Ka~+电极的平均斜率为54±1.4mV(±SD)。K~+电极的平均斜率为57.2±1.4mV(±SD)。Na~+电极的平均检测下限为9.0±1.1×10~(-0)M(±SD),K~+电极的平均检测下限为9.23±0.71×10~(-7)M(±SD)。电极的稳定性能良好,平均寿命约一周。
In this study, we used the ion-selective microelectrodes (ISMs) of Na ~ +, K ~ + and reference cells to detect the dynamic changes of the normal cortex and middle cerebral artery in 14 Wistar rats (Middle cerebralartery occlusion and MCAO, and the activity of Na ~ +, K ~ + and reference ISMs were tested. The experimental results showed that the extracellular Na ~ + in cerebral infarction area decreased rapidly from 140.2 ± 9.3mM (± SD) to 129.7 ± 10.2mM (± SD) in 1-2 minutes after MCAO. While K ~ + increased from a normal mean of 4.29 ± 0.41 mM (± SD) to 6.12 ± 1.00 mM (± SD). The linear range of Na ~ + electrode is in the range of 10 ~ (-5) -10 ~ (-1) M, the linear range of K ~ + electrode is in the range of 10 ~ (-1) -10 ~ (-6) M, The average slope was 54 ± 1.4 mV (± SD). The average slope of K ~ + electrodes was 57.2 ± 1.4 mV (± SD). The average detection limit of Na ~ + electrode was 9.0 ± 1.1 × 10 ~ (-0) M (± SD), and the average detection limit of K + electrode was 9.23 ± 0.71 × 10 -7 M (± SD). Electrode stability is good, the average life expectancy of about a week.