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为寻找肝癌发生过程中较为重要的癌基因，并用于基因诊断作者应用Ｎｏｒｔｈｅｒｎ印迹法分析８例多结节肝癌及相应癌旁组织中的ＩＧＦ－Ⅱ，ｃ－ｍｙｃ，Ｎ－ｒａｓ３种癌基因的表达水平，结果发现３种癌基因在８例癌结节中呈不同程度的过量表达。其中１例的各个癌结节中３个癌基因表达程度基本平行。ＩＧＦ－Ⅱ和ｃ－ｍｙｃ在癌旁组织呈过量表达，其中３例ＩＧＦ－Ⅱ的表达超过癌组织，而Ｎ－ｒａｓ在癌旁组织的表达都接近正常。提示ＩＧＦ－Ⅱ和ｃ－ｍｙｃ基因与肝细胞增生有关，而Ｎ－ｒａｓ过量表达具有诊断意义。
In order to search for the more important oncogene in the process of liver cancer, and use it in gene diagnosis, we used Northern blotting to analyze the expression of IGF-II, c-myc, and N-ras oncogenes in 8 cases of multi-nodular hepatocellular carcinoma and its adjacent tissues. At the expression level, the three oncogenes were found to be overexpressed to varying degrees in 8 cases of cancer nodules. Among the cancer nodules in 1 case, the expression levels of 3 oncogenes were basically parallel. IGF-II and c-myc were over-expressed in adjacent tissues, and 3 of them had more IGF-II expression than cancerous tissues. The expression of N-ras in adjacent tissues was nearly normal. It is suggested that IGF-II and c-myc genes are related to the proliferation of hepatocytes, while the overexpression of N-ras is of diagnostic significance.