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目的:探讨兰溪市农村儿童肠道志贺菌的分离情况及耐药性的情况,为儿童细菌性腹泻的治疗与预防提供依据。方法:采集兰溪市农村在校和不在校的3078名儿童(年龄6~12岁)的新鲜粪便,经粪便细菌培养鉴定,并用KB法进行体外抗菌药物的敏感性检测。结果:在3078名儿童的肠道中分离到志贺菌86株(2.79%),其中痢疾志贺菌17株,占分离菌株的19.8%;福氏志贺菌II 57株,占分离菌株的66.3%;宋内志贺菌12株,占分离菌株的13.9%,鲍氏志贺菌0株。体外抗菌药物敏感性检测显示,86株志贺菌总的耐药率,氨苄西林76.7%、头孢曲松14.0%、亚胺培南0.0%、氯霉素9.3%、四环素75.6%、庆大霉素37.2%、环丙沙星4.7%、复方新诺明81.4%。结论:兰溪市农村的儿童肠道中携带有一定比例的志贺菌,其中以福氏志贺菌为主。体外抗菌药物的敏感性以三代头孢菌素、亚胺培南、环丙沙星较好。
Objective: To investigate the isolation and drug resistance of Shigella flexneri in rural children in Lanxi City, and to provide basis for the treatment and prevention of bacterial diarrhea in children. Methods: Fresh stools of 3078 children (aged 6-12 years old) in rural Lanxi were enrolled in the study. The stool samples were used for bacterial culture of the stool samples. KB method was used to detect the antibacterial activity in vitro. Results: 86 strains (2.79%) of Shigella were isolated from the intestinal tract of 3078 children, of which 17 strains were Shigella dysenteriae, accounting for 19.8% of the isolates; 57 strains of Shigella flexneri II, accounting for 66.3 %; Shigella sonnei 12, accounting for 13.9% of isolates, 0 strains of Shigella spp. In vitro antibacterial susceptibility testing showed that the total drug resistance rate of 86 strains of Shigella was 76.7% of ampicillin, 14.0% of ceftriaxone, 0.0% of imipenem, 9.3% of chloramphenicol, 75.6% of tetracycline, 37.2% prime, 4.7% ciprofloxacin, 81.4% cotrimoxazole. Conclusion: A certain proportion of Shigella is carried in the intestines of children in rural Lanxi, among which Shigella flexneri is the major component. In vitro antibacterial susceptibility to third-generation cephalosporins, imipenem, ciprofloxacin better.