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本文通过增加对人精子低渗透压负荷试验,从形态学观察精子尾部的肿胀程度,从本质上弄清精子膜的功能,用以判断人精子的受精能力。主要就低渗肿胀测定(HOST)和一般精液检查的相关性进行了研究。研究对象为在日本岛根医大妇产科不育门诊治疗的不育夫妇中18名精液检查异常的男子,以6名健康男子的正常精液作对照,进行比较研究。受试者禁欲5日以上,手淫法取精液,在室温中30分钟液化后进行检查。正常精液标准:精液量>2ml、精子密度>60×10~6/ml、精子活动率>60%、畸形精子<15%。 HOST的判断:计算100个以上的精子,并将肿胀精子分成a～g 7型。a型为正常无变化,b～g
In this paper, by increasing the human sperm hypotonic pressure test, morphological observation of the swelling of the sperm tail, to clarify the essence of sperm membrane function, to determine the ability of human sperm fertilization. Mainly on hypotonic swelling test (HOST) and the correlation between the general semen examination. The study was performed on 18 men with abnormal semen from infertile couples treated at Obstetrics and Gynecology Outpatient Department, Shimane University, Japan, and compared with 6 healthy men’s normal semen. Subjects abstinence 5 days or more, masturbation method to take semen, liquefaction after 30 minutes at room temperature for examination. Normal semen standards: semen volume> 2ml, sperm density> 60 × 10 ~ 6 / ml, sperm motility rate> 60%, abnormal sperm <15%. HOST judgment: Calculate more than 100 sperm, and swollen sperm into a ~ g 7 type. a type of normal no change, b ~ g