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用小麦条锈菌条中30号生理小种,对小麦抗病种质小麦-簇毛麦易位系V9128-1和铭贤169的杂交后代进行抗条锈性遗传分析,小麦-簇毛麦易位系V9128-1的抗病性符合1对显性抗条锈病基因控制。并根据F2抗、感病单株分离比例组建抗感池,用SSR技术寻找与抗病基因连锁的分子标记。从121个SSR引物组合中筛选到2个与抗病基因YrV1(暂命名)紧密连锁的微卫星标记Xgwm566和Xgwm376,遗传距离分别为3.6和5.5cM;因此,该抗条锈病基因位于小麦3B染色体短臂上。这2个标记不仅能在小麦-簇毛麦易位系V9128-1中检测到,而且在抗病基因供体亲本簇毛麦中也能检测到。综合抗病基因来源和分子生物学试验结果,可以推断,YrV1很可能是1个来自簇毛麦并与已知抗条锈病基因不同的新基因。
The stripe rust resistance was analyzed by crossing the progeny of wheat stripe rust race No.30 with race V9128-1 and Mingxian169, The resistance of the translocation line V9128-1 conformed to a gene control of dominant stripe rust resistance. The anti-sense pool was constructed according to the ratio of F2 anti-susceptible and susceptible plants, and the molecular markers linked to disease-resistance genes were searched by SSR technique. Two microsatellite markers, Xgwm566 and Xgwm376, closely linked to the YrV1 (tentative name) resistance gene were screened out from 121 SSR primer combinations and their genetic distances were 3.6 and 5.5 cM, respectively. Therefore, the stripe rust resistance gene was located on chromosome 3B Short arm. These two markers can be detected not only in the wheat-cluster mahogany translocation line V9128-1, but also in the cluster-resistant maize host. Based on the origin of the disease-resistance genes and the results of molecular biology tests, it can be concluded that YrV1 is probably a new gene from Hamster and different stripe rust resistance genes.